ArticleJournal of biomedicine & biotechnology2011
Expression of recombinant human coagulation factor VII by the Lizard Leishmania expression system.
Article in Journal of biomedicine & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Heterologous Expression of Human IFNγ and Anti-IL17 Antibody in Leishmania tarentolae Promastigote.Acta parasitologica · 2024Article
- Site-directed mutagenesis of tissue factor pathway inhibitor-binding exosite D60A on factor VII results in a new factor VII variant with lower coagulant activity.Research and practice in thrombosis and haemostasis · 2024Article
- Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules.Human vaccines & immunotherapeutics · 2017Review
- Purification and Characterization of Recombinant Darbepoetin Alfa from Leishmania tarentolae.Molecular biotechnology · 2016Article
- Enhanced recombinant factor VII expression in Chinese hamster ovary cells by optimizing signal peptides and fed-batch medium.Bioengineered · 2016Article
- Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay.Iranian journal of public health · 2015Article
- Cloning and Expression of Leishmania infantum LPG3 Gene by the Lizard Leishmania Expression System.Avicenna journal of medical biotechnology · 2012Article
- Genomic organization of leishmania species.Iranian journal of parasitology · 2011Article
- Preliminary Information of Iranian LizardIranian journal of parasitologyArticle
- Comparing the Yield of Recombinant Human Factor VII Protein Expressed by the rDNA-Promoter with the CMV-Promoter in Iranian LizardIranian journal of parasitologyArticle
- The Influence of Different Culture Media on the Growth and Recombinant Protein Production of Iranian Lizard Leishmania Promastigote.Iranian journal of parasitologyArticle
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9 authors.
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Abstract
The variety of recombinant protein expression systems have been developed as a resource of FVII gene expression. In the current study, the authors used a novel protein expression system based on the Iranian Lizard Leishmania, a trypanosomatid protozoan as a host for expression of FVII. Plasmid containing cDNA encoding full-length human FVII was introduced into Lizard Leishmania and positive transfectants were analyzed by SDS-PAGE and Western blot analysis. Furthermore, biological activity of purified protein was detected by PT assay. The recombinant strain harboring a construct was analyzed for expression of FVII at the mRNA and protein level. Purified rFVII was obtained and in order to confirm the purified compound was in fact rFVII. Western blot analysis was carried out. Clotting time in PT assay was reduced about 30 seconds with the purified rFVII. In Conclusion, this study has demonstrated, for the first time, that Leishmania cells can be used as an expression system for producing recombinant FVII.
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