ArticleThe Journal of biological chemistry2011
Characterization and scope of S-layer protein O-glycosylation in Tannerella forsythia.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 95 citations in OpenAlex.
- Interspecies regulation of virulence through gingipain inhibition by a Tannerella forsythia serpin.EMBO reports · 2026Article
- Uncovering bacterial pseudaminylation with pan-specific antibody tools.Nature chemical biology · 2026Article
- Protein O-glycosylation in the Bacteroidota phylum.FEBS open bio · 2026Review
- Microbial Coaggregation in the Oral Cavity: Molecular Interactions and Current Insights.International journal of molecular sciences · 2025Review
- Deciphering fucosylated protein-linked O-glycans in oral Tannerella serpentiformis: Insights from NMR spectroscopy and glycoproteomics.Glycobiology · 2024Article
- Ultrastructural and glycoproteomic characterization of Prevotella intermedia: Insights into O-glycosylation and outer membrane vesicles.MicrobiologyOpen · 2024Article
- Screening and characterization of aptamers recognizing the periodontal pathogen Tannerella forsythia.FEBS open bio · 2024Article
- The intriguing strategies ofFrontiers in oral health · 2024Article
- A journey with type IX secretion system effectors: selection, transport, processing and activities.Microbiology (Reading, England) · 2023Article
- Multispecies biofilm behavior and host interaction support the association of Tannerella serpentiformis with periodontal health.Molecular oral microbiology · 2023Article
- Primary Structure of Glycans by NMR Spectroscopy.Chemical reviews · 2023Review
- S-layer proteins as immune players: Tales from pathogenic and non-pathogenic bacteria.Current research in microbial sciences · 2023Review
- Modulatory Mechanisms of Pathogenicity inMicroorganisms · 2022Review
- The type IX secretion system: Insights into its function and connection to glycosylation inEngineering microbiology · 2022Review
- A Micrarchaeon Isolate Is Covered by a Proteinaceous S-Layer.Applied and environmental microbiology · 2022Article
- Characterization of the O-Glycoproteome of Porphyromonas gingivalis.Microbiology spectrum · 2022Article
- A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium.The ISME journal · 2022Article
- Glycopeptide-Centric Approaches for the Characterization of Microbial Glycoproteomes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Combination of Structural, Genetic, Phenotypic and Enzymatic Analyses Reveals the Importance of a Predicted Fucosyltransferase to ProteinBiomolecules · 2021Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Cell surface glycosylation is an important element in defining the life of pathogenic bacteria. Tannerella forsythia is a Gram-negative, anaerobic periodontal pathogen inhabiting the subgingival plaque biofilms. It is completely covered by a two-dimensional crystalline surface layer (S-layer) composed of two glycoproteins. Although the S-layer has previously been shown to delay the bacterium's recognition by the innate immune system, we characterize here the S-layer protein O-glycosylation as a potential virulence factor. The T. forsythia S-layer glycan was elucidated by a combination of electrospray ionization-tandem mass spectrometry and nuclear magnetic resonance spectroscopy as an oligosaccharide with the structure 4-Me-β-ManpNAcCONH(2)-(1→3)-[Pse5Am7Gc-(2→4)-]-β-ManpNAcA-(1→4)-[4-Me-α-Galp-(1→2)-]-α-Fucp-(1→4)-[-α-Xylp-(1→3)-]-β-GlcpA-(1→3)-[-β-Digp-(1→2)-]-α-Galp, which is O-glycosidically linked to distinct serine and threonine residues within the three-amino acid motif (D)(S/T)(A/I/L/M/T/V) on either S-layer protein. This S-layer glycan obviously impacts the life style of T. forsythia because increased biofilm formation of an UDP-N-acetylmannosaminuronic acid dehydrogenase mutant can be correlated with the presence of truncated S-layer glycans. We found that several other proteins of T. forsythia are modified with that specific oligosaccharide. Proteomics identified two of them as being among previously classified antigenic outer membrane proteins that are up-regulated under biofilm conditions, in addition to two predicted antigenic lipoproteins. Theoretical analysis of the S-layer O-glycosylation of T. forsythia indicates the involvement of a 6.8-kb gene locus that is conserved among different bacteria from the Bacteroidetes phylum. Together, these findings reveal the presence of a protein O-glycosylation system in T. forsythia that is essential for creating a rich glycoproteome pinpointing a possible relevance for the virulence of this bacterium.
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