ArticleThe Journal of cell biology2011
Arf6 regulates AP-1B-dependent sorting in polarized epithelial cells.
Article in The Journal of cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- The calcium pump PMCA4b promotes epithelial cell polarization and lumen formation.Communications biology · 2025Article
- Multifaceted control of E-cadherin dynamics by Adaptor Protein Complex 1 during epithelial morphogenesis.Molecular biology of the cell · 2022Article
- AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia.Frontiers in cell and developmental biology · 2021Article
- Novel function for AP-1B during cell migration.Molecular biology of the cell · 2020Article
- The small GTPase ARF-1.2 is a regulator of unicellular tube formation in Caenorhabditis elegans.The journal of physiological sciences : JPS · 2019Article
- LRBA in the endomembrane system.Colombia medica (Cali, Colombia) · 2018Review
- ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- EGFR/ARF6 regulation of Hh signalling stimulates oncogenic Ras tumour overgrowth.Nature communications · 2017Article
- The cellular mechanisms that maintain neuronal polarity.Nature reviews. Neuroscience · 2016Review
- MARTX effector cross kingdom activation by Golgi-associated ADP-ribosylation factors.Cellular microbiology · 2016Article
- Heike Folsch: Peeling back the layers.The Journal of cell biology · 2016Article
- Article
- Adaptor protein complexes and intracellular transport.Bioscience reports · 2014Review
- Adaptor proteins involved in polarized sorting.The Journal of cell biology · 2014Review
- Golgi in copper homeostasis: a view from the membrane trafficking field.Histochemistry and cell biology · 2013Review
- Mutations in ap1b1 cause mistargeting of the Na(+)/K(+)-ATPase pump in sensory hair cells.PloS one · 2013Article
- Role of membrane traffic in the generation of epithelial cell asymmetry.Nature cell biology · 2012Review
- RAB-10-GTPase-mediated regulation of endosomal phosphatidylinositol-4,5-bisphosphate.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Polarization and myelination in myelinating glia.ISRN neurology · 2012Article
- The role of secretory and endocytic pathways in the maintenance of cell polarity.Essays in biochemistry · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The epithelial cell-specific clathrin adaptor complex AP-1B facilitates the sorting of various transmembrane proteins from recycling endosomes (REs) to the basolateral plasma membrane. Despite AP-1B's clear importance in polarized epithelial cells, we still do not fully understand how AP-1B orchestrates basolateral targeting. Here we identify the ADP-ribosylation factor 6 (Arf6) as an important regulator of AP-1B. We show that activated Arf6 pulled down AP-1B in vitro. Furthermore, interfering with Arf6 function through overexpression of dominant-active Arf6Q67L or dominant-negative Arf6D125N, as well as depletion of Arf6 with short hairpin RNA (shRNA), led to apical missorting of AP-1B-dependent cargos. In agreement with these data, we found that Arf6 colocalized with AP-1B and transferrin receptor (TfnR) in REs. In addition, we observed specific recruitment of AP-1B into Arf6-induced membrane ruffles in nonpolarized cells. We conclude that activated Arf6 directs membrane recruitment of AP-1B, thus regulating AP-1B's functions in polarized epithelial cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.