ArticleNucleic acids research2012
DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- DNA-RNA hybrid G-quadruplex tends to form near the 3' end of telomere overhang.Biophysical journal · 2022Article
- Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.PLoS genetics · 2022Article
- Telomere dysfunction implicates POT1 in patients with idiopathic pulmonary fibrosis.The Journal of experimental medicine · 2022Article
- POT1-TPP1 binding stabilizes POT1, promoting efficient telomere maintenance.Computational and structural biotechnology journal · 2022Article
- Review
- POT1-TPP1 differentially regulates telomerase via POT1 His266 and as a function of single-stranded telomere DNA length.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Dynamics of human telomerase recruitment depend on template-telomere base pairing.Molecular biology of the cell · 2018Article
- Structural Basis for Shelterin Bridge Assembly.Molecular cell · 2017Article
- Structural and functional analysis of the human POT1-TPP1 telomeric complex.Nature communications · 2017Article
- Telomere Recognition and Assembly Mechanism of Mammalian Shelterin.Cell reports · 2017Article
- Tying up the Ends: Plasticity in the Recognition of Single-Stranded DNA at Telomeres.Biochemistry · 2016Article
- The telomeric protein Pot1 from Schizosaccharomyces pombe binds ssDNA in two modes with differing 3' end availability.Nucleic acids research · 2014Article
- The oligonucleotide/oligosaccharide-binding fold motif is a poly(ADP-ribose)-binding domain that mediates DNA damage response.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Tpz1 controls a telomerase-nonextendible telomeric state and coordinates switching to an extendible state via Ccq1.Genes & development · 2013Article
- Coordinated interactions of multiple POT1-TPP1 proteins with telomere DNA.The Journal of biological chemistry · 2013Article
- Finding the end: recruitment of telomerase to telomeres.Nature reviews. Molecular cell biology · 2013Review
- Practical strategies for the evaluation of high-affinity protein/nucleic acid interactions.Journal of nucleic acids investigation · 2013Article
- Seryl tRNA synthetase cooperates with POT1 to regulate telomere length and cellular senescence.Signal transduction and targeted therapyArticle
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Eukaryotic chromosome ends are protected from illicit DNA joining by protein-DNA complexes called telomeres. In most studied organisms, telomeric DNA is composed of multiple short G-rich repeats that end in a single-stranded tail that is protected by the protein POT1. Mammalian POT1 binds two telomeric repeats as a monomer in a sequence-specific manner, and discriminates against RNA of telomeric sequence. While addressing the RNA discrimination properties of SpPot1, the POT1 homolog in Schizosaccharomyces pombe, we found an unanticipated ssDNA-binding mode in which two SpPot1 molecules bind an oligonucleotide containing two telomeric repeats. DNA binding seems to be achieved via binding of the most N-terminal OB domain of each monomer to each telomeric repeat. The SpPot1 dimer may have evolved to accommodate the heterogeneous spacers that occur between S. pombe telomeric repeats, and it also has implications for telomere architecture. We further show that the S. pombe telomeric protein Tpz1, like its mammalian homolog TPP1, increases the affinity of Pot1 for telomeric single-stranded DNA and enhances the discrimination of Pot1 against RNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.