ArticleNucleic acids research2012
Opening-closing dynamics of the mitochondrial transcription pre-initiation complex.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Mitochondrial Transcription of Entomopathogenic Fungi Reveals Evolutionary Aspects of Mitogenomes.Frontiers in microbiology · 2022Article
- Cryo-EM Structures Reveal Transcription Initiation Steps by Yeast Mitochondrial RNA Polymerase.Molecular cell · 2021Article
- Structure, mechanism, and regulation of mitochondrial DNA transcription initiation.The Journal of biological chemistry · 2020Review
- The dynamic landscape of transcription initiation in yeast mitochondria.Nature communications · 2020Article
- A DNA-Binding Bromodomain-Containing Protein Interacts with and Reduces Rx1-Mediated Immune Response to Potato Virus X.Plant communications · 2020Article
- The C-terminal tail of the yeast mitochondrial transcription factor Mtf1 coordinates template strand alignment, DNA scrunching and timely transition into elongation.Nucleic acids research · 2020Article
- Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes.Nature communications · 2019Article
- The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor.The Journal of biological chemistry · 2018Article
- Conformational heterogeneity and bubble dynamics in single bacterial transcription initiation complexes.Nucleic acids research · 2018Article
- Human mitochondrial transcription factors TFAM and TFB2M work synergistically in promoter melting during transcription initiation.Nucleic acids research · 2017Article
- Direct evidence for sequence-dependent attraction between double-stranded DNA controlled by methylation.Nature communications · 2016Article
- The Potato Nucleotide-binding Leucine-rich Repeat (NLR) Immune Receptor Rx1 Is a Pathogen-dependent DNA-deforming Protein.The Journal of biological chemistry · 2015Article
- Allostery through protein-induced DNA bubbles.Scientific reports · 2015Article
- Interactions of the yeast mitochondrial RNA polymerase with the +1 and +2 promoter bases dictate transcription initiation efficiency.Nucleic acids research · 2014Article
- Article
- Single-molecule nanometry for biological physics.Reports on progress in physics. Physical Society (Great Britain) · 2013Review
- Transcription factor-dependent DNA bending governs promoter recognition by the mitochondrial RNA polymerase.The Journal of biological chemistry · 2011Article
- Fluorescent methods to study transcription initiation and transition into elongation.Experientia supplementum (2012)Review
- Mechanism of transcription initiation by the yeast mitochondrial RNA polymerase.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Promoter recognition and local melting of DNA are key steps of transcription initiation catalyzed by RNA polymerase and initiation factors. From single molecule fluorescence resonance energy transfer studies of the yeast (Saccharomyces cerevisiae) mitochondrial RNA polymerase Rpo41 and its transcription factor Mtf1, we show that the pre-initiation complex is highly dynamic and undergoes repetitive opening-closing transitions that are modulated by Mtf1 and ATP. We found that Rpo41 alone has the intrinsic ability to bend the promoter but only very briefly. Mtf1 enhances bending/opening transition and suppresses closing transition, indicating its dual roles of nucleating promoter opening and stabilizing the open state. The cognate initiating ATP prolongs the lifetime of the open state, plausibly explaining the 'ATP sensing mechanism' suggested for the system. We discovered short-lived opening trials upon initial binding of Rpo41-Mtf1 before the establishment of the opening/closing equilibrium, which may aid in promoter selection before the formation of stable pre-initiation complex. The dynamics of open complex formation provides unique insights into the interplay between RNA polymerase and transcription factors in regulating initiation.
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