ArticleJournal of neuroscience methods2012
FACS purification of immunolabeled cell types from adult rat brain.
Article in Journal of neuroscience methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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Who cites it
80 citing papers in PubMed, 136 citations in OpenAlex.
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- A validated FACS-based protocol for the isolation of neuronal and non-neuronal cells from frozen adult brain, suitable for integrated gene and protein analysis.Pharmacological reports : PR · 2026Article
- Pyk2 dysregulation in temporal lobe epilepsy: insights from human resected tissues and pharmacological modulation in a rodent model.Journal of neural transmission (Vienna, Austria : 1996) · 2026Article
- Writing the Engram: Epigenetic Mechanisms of Memory Allocation.Journal of neurochemistry · 2025Review
- CREB3 gain of function variants protect against ALS.Nature communications · 2025Article
- VLK drives extracellular phosphorylation of EphB2 to govern the EphB2-NMDAR interaction and injury-induced pain.bioRxiv : the preprint server for biology · 2024Article
- Aberrant gene expression yet undiminished retinal ganglion cell genesis in iPSC-derived models of optic nerve hypoplasia.Ophthalmic genetics · 2024Article
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- Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors ofiScience · 2023Article
- Role of Piriform Cortex and Its Afferent Projections in Relapse to Fentanyl Seeking after Food Choice-Induced Voluntary Abstinence.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Hierarchical deconvolution for extensive cell type resolution in the human brain using DNA methylation.Frontiers in neuroscience · 2023Article
- Isolation of human pluripotent stem cell-derived sensory neuron subtypes by immunopanning.Frontiers in cell and developmental biology · 2023Article
- Sequential Isolation of Microglia and Astrocytes from Young and Aged Adult Mouse Brains for Downstream Transcriptomic Analysis.Methods and protocols · 2022Article
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- Optimized Protocol for Proportionate CNS Cell Retrieval as a Versatile Platform for Cellular and Molecular Phenomapping in Aging and Neurodegeneration.International journal of molecular sciences · 2022Article
- Direct FACS Isolation of Neural Stem/Progenitor Lineages from the Adult Brain.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Limb-clasping, cognitive deficit and increased vulnerability to kainic acid-induced seizures in neuronal glycosylphosphatidylinositol deficiency mouse models.Human molecular genetics · 2021Article
- Cellular, circuit and transcriptional framework for modulation of itch in the central amygdala.eLife · 2021Article
- Investigating the effects of chronic perinatal alcohol and combined nicotine and alcohol exposure on dopaminergic and non-dopaminergic neurons in the VTA.Scientific reports · 2021Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Molecular analysis of brain tissue is greatly complicated by having many different classes of neurons and glia interspersed throughout the brain. Fluorescence-activated cell sorting (FACS) has been used to purify selected cell types from brain tissue. However, its use has been limited to brain tissue from embryos or transgenic mice with promoter-driven reporter genes. To overcome these limitations, we developed a FACS procedure for dissociating intact cell bodies from adult wild-type rat brains and sorting them using commercially available antibodies against intracellular and extracellular proteins. As an example, we isolated neurons using a NeuN antibody and confirmed their identity using microarray and real time PCR of mRNA from the sorted cells. Our FACS procedure allows rapid, high-throughput, quantitative assays of molecular alterations in identified cell types with widespread applications in neuroscience.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.