ArticleMicrobial cell factories2011
Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid.
Article in Microbial cell factories, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 64 citations in OpenAlex.
- Enhanced bioproduction and processing of mandelic acid enantiomers: towards a sustainable platform for high-value pharmaceutical and polymer applications.Biotechnology for biofuels and bioproducts · 2025Article
- EngineeringBiodesign research · 2025Article
- Biosynthesis Strategies and Application Progress of Mandelic Acid Based on Biomechanical Properties.Microorganisms · 2025Review
- Production of aromatic amino acids and their derivatives by Escherichia coli and Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Review
- Systematic engineering enables efficient biosynthesis of L-phenylalanine in E. coli from inexpensive aromatic precursors.Microbial cell factories · 2024Article
- Co-production of biofuel, bioplastics and biochemicals during extended fermentation of Halomonas bluephagenesis.Microbial biotechnology · 2023Article
- Separation of Mandelic Acid by a Reactive Extraction Method Using Tertiary Amine in Different Organic Diluents.Molecules (Basel, Switzerland) · 2022Article
- Vitreoscilla hemoglobin enhances the catalytic performance of industrial oxidases in vitro.Applied microbiology and biotechnology · 2022Article
- Construction of recombinant Escherichia coli for production of L-phenylalanine-derived compounds.World journal of microbiology & biotechnology · 2021Review
- Production of (R)-mandelic acid from styrene, L-phenylalanine, glycerol, or glucose via cascade biotransformations.Bioresources and bioprocessing · 2021Article
- Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers.Metabolic engineering · 2020Article
- Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids.Frontiers in bioengineering and biotechnology · 2020Review
- Metabolic engineering of microorganisms for production of aromatic compounds.Microbial cell factories · 2019Review
- Bacterial bifunctional chorismate mutase-prephenate dehydratase PheA increases flux into the yeast phenylalanine pathway and improves mandelic acid production.Metabolic engineering communications · 2018Article
- Metabolic Engineering of the Shikimate Pathway for Production of Aromatics and Derived Compounds-Present and Future Strain Construction Strategies.Frontiers in bioengineering and biotechnology · 2018Article
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2018Article
- Metabolic engineering of indole pyruvic acid biosynthesis in Escherichia coli with tdiD.Microbial cell factories · 2017Article
- The Need for Integrated Approaches in Metabolic Engineering.Cold Spring Harbor perspectives in biology · 2016Review
- Engineering Escherichia coli for production of 4-hydroxymandelic acid using glucose-xylose mixture.Microbial cell factories · 2016Article
- EngineeringMetabolic engineering communications · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMandelic acid (MA), an important component in pharmaceutical syntheses, is currently produced exclusively via petrochemical processes. Growing concerns over the environment and fossil energy costs have inspired a quest to develop alternative routes to MA using renewable resources. Herein we report the first direct route to optically pure MA from glucose via genetic modification of the L-phenylalanine pathway in E. coli.
resultsThe introduction of hydroxymandelate synthase (HmaS) from Amycolatopsis orientalis into E. coli led to a yield of 0.092 g/L S-MA. By combined deletion of competing pathways, further optimization of S-MA production was achieved, and the yield reached 0.74 g/L within 24 h. To produce R-MA, hydroxymandelate oxidase (Hmo) from Streptomyces coelicolor and D-mandelate dehydrogenase (DMD) from Rhodotorula graminis were co-expressed in an S-MA-producing strain, and the resulting strain was capable of producing 0.68 g/L R-MA. Finally, phenylpyruvate feeding experiments suggest that HmaS is a potential bottleneck to further improvement in yields.
conclusionsWe have constructed E. coli strains that successfully accomplished the production of S- and R-MA directly from glucose. Our work provides the first example of the completely fermentative production of S- and R-MA from renewable feedstock.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.