ArticleMethods in molecular biology (Clifton, N.J.)2011
Probing DNA topology using tethered particle motion.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination.Micromachines · 2018Review
- Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme Dynamics.Methods in enzymology · 2017Article
- Article
- High-throughput single-molecule studies of protein-DNA interactions.FEBS letters · 2014Review
- Enhanced tethered-particle motion analysis reveals viscous effects.Biophysical journal · 2014Article
- MADS domain transcription factors mediate short-range DNA looping that is essential for target gene expression in Arabidopsis.The Plant cell · 2013Article
- Single molecule analysis of DNA wrapping and looping by a circular 14mer wheel of the bacteriophage 186 CI repressor.Nucleic acids research · 2013Article
- Determination of the number of proteins bound non-specifically to DNA.Journal of physics. Condensed matter : an Institute of Physics journal · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Transcription factors mediate the formation of nucleoprotein complexes that are critical for efficient regulation of epigenetic switches. In these complexes, DNA is frequently bent or looped by the protein; other times, strong interactions lead the DNA to fully wrap the regulatory protein(s). The equilibrium between the bending, looping, full and partial wrapping of DNA governs the level of transcriptional regulation and is tuned by biophysical parameters. Characterization of the structure, kinetics, and thermodynamics of formation of such nucleoprotein complexes is fundamental to the understanding of the molecular mechanisms that underlie the operation of the genetic switches controlled by them. Here, we describe in detail how to perform tethered particle motion experiments aimed at understanding how protein-DNA interactions influence the formation and breakdown of these regulatory complexes.
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Registered trials
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