Evidence map›Paper›PMID 21909887›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2011

Exploring protein superstructures and dynamics in live bacterial cells using single-molecule and superresolution imaging.

Julie S Biteen, Lucy Shapiro, W E Moerner

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
5.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Julie S BiteenDepartment of Chemistry, University of Michigan, Ann Arbor, MI, USA. jsbiteen@umich.edu
Lucy Shapiro
W E Moerner
Stanford University · USUniversity of Michigan–Ann Arbor · US

Funding

REGULATION OF DIFFERENTIATION IN CAULOBACTERR01GM032506 · NIGMS · YESHIVA UNIVERSITY · PI SHAPIRO, LUCILLE · 1985 to 2014
$7.1M
Subcellular architecture of regulatory protein complexes at the bacterial poleR01GM086196 · NIGMS · STANFORD UNIVERSITY · PI MOERNER, WILLIAM E, SHAPIRO, LUCILLE · 2008 to 2015
$4.7M
DEVELOPMENT CONTROL OF DNA REPLICATION IN CAULOBACTERR01GM051426 · NIGMS · STANFORD UNIVERSITY · PI SHAPIRO, LUCILLE · 1994 to 2013
$3.8M
Single-Molecule Fluorophores for Cellular Imaging(RMI)P20HG003638 · NHGRI · STANFORD UNIVERSITY · PI MOERNER, WILLIAM E · 2004 to 2008
$3.0M
Three-Dimensional Superresolution Imaging in Living Cells Using Single-Molecule AR01GM085437 · NIGMS · STANFORD UNIVERSITY · PI MOERNER, WILLIAM E · 2008 to 2015
$2.1M
NHGRI NIH HHS P20 HG003638NHGRI NIH HHS P20-HG003638NIGMS NIH HHS R01 GM032506NIGMS NIH HHS R01 GM051426NIGMS NIH HHS R01 GM085437NIGMS NIH HHS R01-GM085437NIGMS NIH HHS R01 GM086196NIGMS NIH HHS R01-GM086196NIGMS NIH HHS R01-GM32506NIGMS NIH HHS R01-GM51426
6 · The paper itself

Abstract

Single-molecule imaging enables biophysical measurements devoid of ensemble averaging, gives enhanced spatial resolution beyond the optical diffraction limit, and enables superresolution reconstruction of structures beyond the diffraction limit. This work summarizes how single-molecule and superresolution imaging can be applied to the study of protein dynamics and superstructures in live Caulobacter crescentus cells to illustrate the power of these methods in bacterial imaging. Based on these techniques, the diffusion coefficient and dynamics of the histidine protein kinase PleC, the localization behavior of the polar protein PopZ, and the treadmilling behavior and protein superstructure of the structural protein MreB are investigated with sub-40-nm spatial resolution, all in live cells.

Indexed as

Bacterial ProteinsCaulobacter crescentusModels, TheoreticalMolecular ImagingBacterial Proteins

Identifiers

PMID21909887
PMCPMC3702733
OpenAlexW2119086365

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.