ArticleNature biotechnology2011
A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching.
Article in Nature biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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Who cites it
109 citing papers in PubMed.
- Recent developments of fluorescent proteins and biosensors driven by cross-disciplinary insights.Nature methods · 2026Review
- Time-lapse RESOLFT nanoscopy empowered by deep learning.Nature communications · 2026Article
- Bright monomeric fluorescent protein elite-niRFP704 for two-channel near-infrared STED nanoscopy.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Dynamics and Mechanism of Off- to On-Switching in Dreiklang a Decoupled Reversibly Switchable Fluorescent Protein.Angewandte Chemie (International ed. in English) · 2026Article
- A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Ultrafast photophysics of a positive reversibly switchable fluorescent protein.Chemical science · 2025Article
- 1000 fold Ultra-Photosensitized Fluorescent Protein Mimics Toward Photocatalytic Proximity Labeling and Proteomic Profiling Functions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- In situ architecture of the human prohibitin complex.Nature cell biology · 2025Article
- Photoactivatable Blue Fluorescent Protein.ACS omega · 2024Article
- Multiple dyes applications for fluorescent convertible polymer capsules as macrophages tracking labels.Heliyon · 2024Article
- Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag.Communications biology · 2024Article
- The role of the correlated motion(s) of the chromophore in photoswitching of green and red forms of the photoconvertible fluorescent protein mSAASoti.Scientific reports · 2024Article
- Sub-Diffraction Readout Method of High-Capacity Optical Data Storage Based on Polarization Modulation.Nanomaterials (Basel, Switzerland) · 2024Article
- Review
- Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism.Journal of the American Chemical Society · 2023Article
- Article
- QM/MM Modeling of the Flavin Functionalization in the RutA Monooxygenase.Molecules (Basel, Switzerland) · 2023Article
- Modeling Light-Induced Chromophore Hydration in the Reversibly Photoswitchable Fluorescent Protein Dreiklang.Molecules (Basel, Switzerland) · 2023Article
- Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application.Communications biology · 2022Article
49 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photoswitchable fluorescent proteins have enabled new approaches for imaging cells, but their utility has been limited either because they cannot be switched repeatedly or because the wavelengths for switching and fluorescence imaging are strictly coupled. We report a bright, monomeric, reversibly photoswitchable variant of GFP, Dreiklang, whose fluorescence excitation spectrum is decoupled from that for optical switching. Reversible on-and-off switching in living cells is accomplished at illumination wavelengths of ∼365 nm and ∼405 nm, respectively, whereas fluorescence is elicited at ∼515 nm. Mass spectrometry and high-resolution crystallographic analysis of the same protein crystal in the photoswitched on- and off-states demonstrate that switching is based on a reversible hydration/dehydration reaction that modifies the chromophore. The switching properties of Dreiklang enable far-field fluorescence nanoscopy in living mammalian cells using both a coordinate-targeted and a stochastic single molecule switching approach.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.