ArticleApplied and environmental microbiology2011
Partial genome assembly for a candidate division OP11 single cell from an anoxic spring (Zodletone Spring, Oklahoma).
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 65 citations in OpenAlex.
- Article
- Amynthas corticis genome reveals molecular mechanisms behind global distribution.Communications biology · 2021Article
- Peatland Microbial Community Composition Is Driven by a Natural Climate Gradient.Microbial ecology · 2020Article
- Bacterial Single Cell Whole Transcriptome Amplification in Microfluidic Platform Shows Putative Gene Expression Heterogeneity.Analytical chemistry · 2019Article
- Redundancy in Anaerobic Digestion Microbiomes during Disturbances by the Antibiotic Monensin.Applied and environmental microbiology · 2018Article
- Optofluidic Single-Cell Genome Amplification of Sub-micron Bacteria in the Ocean Subsurface.Frontiers in microbiology · 2018Article
- Assessment of Microbial Community Dynamics in River Bank Filtrate Using High-Throughput Sequencing and Flow Cytometry.Frontiers in microbiology · 2018Article
- Impact of Distribution and Network Flushing on the Drinking Water Microbiome.Frontiers in microbiology · 2018Article
- Single cell sequencing: a distinct new field.Clinical and translational medicine · 2017Review
- Analysis of single-cell genome sequences of bacteria and archaea.Emerging topics in life sciences · 2017Article
- Novel approaches in function-driven single-cell genomics.FEMS microbiology reviews · 2017Article
- Global Distribution Patterns and Pangenomic Diversity of the Candidate Phylum "Latescibacteria" (WS3).Applied and environmental microbiology · 2017Article
- Illumina-based analysis of the rhizosphere microbial communities associated with healthy and wilted Lanzhou lily (Lilium davidii var. unicolor) plants grown in the field.World journal of microbiology & biotechnology · 2016Article
- Single-cell genome sequencing: current state of the science.Nature reviews. Genetics · 2016Review
- Phylogeny and physiology of candidate phylum 'Atribacteria' (OP9/JS1) inferred from cultivation-independent genomics.The ISME journal · 2016Article
- Archaeal and bacterial communities across a chronosequence of drained lake basins in Arctic Alaska.Scientific reports · 2015Article
- Spatiotemporal analysis of bacterial diversity in sediments of Sundarbans using parallel 16S rRNA gene tag sequencing.Microbial ecology · 2015Article
- The microbial community of a passive biochemical reactor treating arsenic, zinc, and sulfate-rich seepage.Frontiers in bioengineering and biotechnology · 2015Article
- Recent advances in genomic DNA sequencing of microbial species from single cells.Nature reviews. Genetics · 2014Review
- Advances in high-throughput single-cell microtechnologies.Current opinion in biotechnology · 2014Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Members of candidate division OP11 are widely distributed in terrestrial and marine ecosystems, yet little information regarding their metabolic capabilities and ecological role within such habitats is currently available. Here, we report on the microfluidic isolation, multiple-displacement-amplification, pyrosequencing, and genomic analysis of a single cell (ZG1) belonging to candidate division OP11. Genome analysis of the ∼270-kb partial genome assembly obtained showed that it had no particular similarity to a specific phylum. Four hundred twenty-three open reading frames were identified, 46% of which had no function prediction. In-depth analysis revealed a heterotrophic lifestyle, with genes encoding endoglucanase, amylopullulanase, and laccase enzymes, suggesting a capacity for utilization of cellulose, starch, and, potentially, lignin, respectively. Genes encoding several glycolysis enzymes as well as formate utilization were identified, but no evidence for an electron transport chain was found. The presence of genes encoding various components of lipopolysaccharide biosynthesis indicates a Gram-negative bacterial cell wall. The partial genome also provides evidence for antibiotic resistance (β-lactamase, aminoglycoside phosphotransferase), as well as antibiotic production (bacteriocin) and extracellular bactericidal peptidases. Multiple mechanisms for stress response were identified, as were elements of type I and type IV secretion systems. Finally, housekeeping genes identified within the partial genome were used to demonstrate the OP11 affiliation of multiple hitherto unclassified genomic fragments from multiple database-deposited metagenomic data sets. These results provide the first glimpse into the lifestyle of a member of a ubiquitous, yet poorly understood bacterial candidate division.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.