ArticleApplied and environmental microbiology2011
The RNA polymerase omega factor RpoZ is regulated by PhoP and has an important role in antibiotic biosynthesis and morphological differentiation in Streptomyces coelicolor.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Prodigiosin: unveiling the crimson wonder - a comprehensive journey from diverse bioactivity to synthesis and yield enhancement.Frontiers in microbiology · 2024Review
- Article
- Article
- Two-Component Systems ofInternational journal of molecular sciences · 2022Review
- Comparative Proteomic Analysis of Transcriptional and Regulatory Proteins Abundances inInternational journal of molecular sciences · 2022Article
- High-Throughput Transcriptional Characterization of Regulatory Sequences from Bacterial Biosynthetic Gene Clusters.ACS synthetic biology · 2021Article
- Rational engineering of a modular bacterial CRISPR-Cas activation platform with expanded target range.Nucleic acids research · 2021Article
- The Balance Metabolism Safety Net: Integration of Stress Signals by Interacting Transcriptional Factors inFrontiers in microbiology · 2019Review
- Association of ω with the C-Terminal Region of the β' Subunit Is Essential for Assembly of RNA Polymerase in Mycobacterium tuberculosis.Journal of bacteriology · 2018Article
- The master regulator PhoP coordinates phosphate and nitrogen metabolism, respiration, cell differentiation and antibiotic biosynthesis: comparison in Streptomyces coelicolor and Streptomyces avermitilis.The Journal of antibiotics · 2017Review
- Acclimation to High COPlant physiology · 2017Article
- The ω Subunit Governs RNA Polymerase Stability and Transcriptional Specificity in Staphylococcus aureus.Journal of bacteriology · 2017Article
- Small things considered: the small accessory subunits of RNA polymerase in Gram-positive bacteria.FEMS microbiology reviews · 2015Review
- The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria.Nucleic acids research · 2014Article
- Triggers and cues that activate antibiotic production by actinomycetes.Journal of industrial microbiology & biotechnology · 2014Review
- Interplay between genetic regulation of phosphate homeostasis and bacterial virulence.Virulence · 2014Review
- The ω subunit of RNA polymerase is essential for thermal acclimation of the cyanobacterium Synechocystis sp. PCC 6803.PloS one · 2014Article
- Two-component systems in Streptomyces: key regulators of antibiotic complex pathways.Microbial cell factories · 2013Review
- Molecular regulation of antibiotic biosynthesis in streptomyces.Microbiology and molecular biology reviews : MMBR · 2013Review
- Identification of different promoters in the absA1-absA2 two-component system, a negative regulator of antibiotic production in Streptomyces coelicolor.Molecular genetics and genomics : MGG · 2013Article
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7 authors.
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Abstract
The RNA polymerase (RNAP) omega factor (ω) forms a complex with the α₂ββ' core of this enzyme in bacteria. We have characterized the rpoZ gene of Streptomyces coelicolor, which encodes a small protein (90 amino acids) identified as the omega factor. Deletion of the rpoZ gene resulted in strains with a slightly reduced growth rate, although they were still able to sporulate. The biosynthesis of actinorhodin and, particularly, that of undecylprodigiosin were drastically reduced in the ΔrpoZ strain, suggesting that expression of these secondary metabolite biosynthetic genes is dependent upon the presence of RpoZ in the RNAP complex. Complementation of the ΔrpoZ mutant with the wild-type rpoZ allele restored both phenotype and antibiotic production. Interestingly, the rpoZ gene contains a PHO box in its promoter region. DNA binding assays showed that the phosphate response regulator PhoP binds to such a region. Since luciferase reporter studies showed that rpoZ promoter activity was increased in a ΔphoP background, it can be concluded that rpoZ is controlled negatively by PhoP, thus connecting phosphate depletion regulation with antibiotic production and morphological differentiation in Streptomyces.
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