ArticleApplied and environmental microbiology2011
In vitro detection and quantification of botulinum neurotoxin type e activity in avian blood.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Diagnosis of botulism in mammals aided by toxin ELISA and C and D gene RT-PCRs with an emphasis on farm animals.Veterinary research communications · 2024Article
- Confirmation of botulism diagnosis in Australian bird samples by ELISA and RT rtPCR.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- The use of the dynamic weight bearing test to assess the effects of acute, intramuscularly administered botulinum neurotoxin type A1 in rats.Toxicon: X · 2020Article
- Docking Simulation and Sandwich Assay for Aptamer-Based Botulinum Neurotoxin Type C Detection.Biosensors · 2020Article
- Sensitive detection of type G botulinum neurotoxin through Endopep-MS peptide substrate optimization.Analytical and bioanalytical chemistry · 2019Article
- Further optimization of peptide substrate enhanced assay performance for BoNT/A detection by MALDI-TOF mass spectrometry.Analytical and bioanalytical chemistry · 2017Article
- An optical biosensor assay for rapid dual detection of Botulinum neurotoxins A and E.Scientific reports · 2015Article
- Optimization of peptide substrates for botulinum neurotoxin E improves detection sensitivity in the Endopep-MS assay.Analytical biochemistry · 2015Article
- Isolation and quantification of botulinum neurotoxin from complex matrices using the BoTest matrix assays.Journal of visualized experiments : JoVE · 2014Article
- Improved detection of botulinum neurotoxin serotype A by Endopep-MS through peptide substrate modification.Analytical biochemistry · 2013Article
- Development of a Cell-Based Functional Assay for the Detection of Clostridium botulinum Neurotoxin Types A and E.International journal of microbiology · 2013Article
- Simultaneous and sensitive detection of six serotypes of botulinum neurotoxin using enzyme-linked immunosorbent assay-based protein antibody microarrays.Analytical biochemistry · 2012Article
- Detection of botulinum neurotoxin serotype A, B, and F proteolytic activity in complex matrices with picomolar to femtomolar sensitivity.Applied and environmental microbiology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Botulinum neurotoxin serotype E (BoNT/E) outbreaks in the Great Lakes region cause large annual avian mortality events, with an estimated 17,000 bird deaths reported in 2007 alone. During an outbreak investigation, blood collected from bird carcasses is tested for the presence of BoNT/E using the mouse lethality assay. While sensitive, this method is labor-intensive and low throughput and can take up to 7 days to complete. We developed a rapid and sensitive in vitro assay, the BoTest Matrix E assay, that combines immunoprecipitation with high-affinity endopeptidase activity detection by Förster resonance energy transfer (FRET) to rapidly quantify BoNT/E activity in avian blood with detection limits comparable to those of the mouse lethality assay. On the basis of the analysis of archived blood samples (n = 87) collected from bird carcasses during avian mortality investigations, BoTest Matrix E detected picomolar quantities of BoNT/E following a 2-h incubation and femtomolar quantities of BoNT/E following extended incubation (24 h) with 100% diagnostic specificity and 91% diagnostic sensitivity.
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