ArticleNucleic acids research2012
dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- Enhanced RNAi does not provide efficient innate antiviral immunity in mice.Nucleic acids research · 2025Article
- Functional canonical RNAi in mice expressing a truncated Dicer isoform and long dsRNA.EMBO reports · 2024Article
- Structural and functional basis of mammalian microRNA biogenesis by Dicer.Molecular cell · 2022Article
- Endogenous Double-Stranded RNA.Non-coding RNA · 2021Review
- VHS, US3 and UL13 viral tegument proteins are required for Herpes Simplex Virus-Induced modification of protein kinase R.Scientific reports · 2020Article
- Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes.PLoS genetics · 2019Article
- Toll-like receptor 2 expression on c-kitNature communications · 2019Article
- Crosstalk Between Mammalian Antiviral Pathways.Non-coding RNA · 2019Review
- Main constraints for RNAi induced by expressed long dsRNA in mouse cells.Life science alliance · 2019Article
- Influenza A virus-derived siRNAs increase in the absence of NS1 yet fail to inhibit virus replication.RNA (New York, N.Y.) · 2018Article
- A-to-I RNA Editing in the Earliest-Diverging Eumetazoan Phyla.Molecular biology and evolution · 2017Article
- Long non-coding RNA exchange during the oocyte-to-embryo transition in mice.DNA research : an international journal for rapid publication of reports on genes and genomes · 2017Article
- Double Stranded RNA in Human Seminal Plasma.Frontiers in genetics · 2017Article
- RECQ5 helicase promotes resolution of conflicts between replication and transcription in human cells.The Journal of cell biology · 2016Article
- Production of small RNAs by mammalian Dicer.Pflugers Archiv : European journal of physiology · 2016Review
- Production of functional small interfering RNAs by an amino-terminal deletion mutant of human Dicer.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Viruses and RNA interference: issues and controversies.Journal of virology · 2014Review
- Article
- An oocyte-specific ELAVL2 isoform is a translational repressor ablated from meiotically competent antral oocytes.Cell cycle (Georgetown, Tex.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Double-stranded RNA (dsRNA) can enter different pathways in mammalian cells, including sequence-specific RNA interference (RNAi), sequence-independent interferon (IFN) response and editing by adenosine deaminases. To study the routing of dsRNA to these pathways in vivo, we used transgenic mice ubiquitously expressing from a strong promoter, an mRNA with a long hairpin in its 3'-UTR. The expressed dsRNA neither caused any developmental defects nor activated the IFN response, which was inducible only at high expression levels in cultured cells. The dsRNA was poorly processed into siRNAs in somatic cells, whereas, robust RNAi effects were found in oocytes, suggesting that somatic cells lack some factor(s) facilitating siRNA biogenesis. Expressed dsRNA did not cause transcriptional silencing in trans. Analysis of RNA editing revealed that a small fraction of long dsRNA is edited. RNA editing neither prevented the cytoplasmic localization nor processing into siRNAs. Thus, a long dsRNA structure is well tolerated in mammalian cells and is mainly causing a robust RNAi response in oocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.