ArticleJournal of structural biology2011
Correlative 3D imaging of whole mammalian cells with light and electron microscopy.
Article in Journal of structural biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Cell Observation and Analysis with a Three-Dimensional Optical Wave Field Microscope.Biosensors · 2025Article
- Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- C. elegans pronuclei fuse after fertilization through a novel membrane structure.The Journal of cell biology · 2020Article
- Semi-automated 3D segmentation of human skeletal muscle using Focused Ion Beam-Scanning Electron Microscopic images.Journal of structural biology · 2019Article
- Diverted recycling-Shigella subversion of Rabs.Small GTPases · 2018Review
- FluoEM, virtual labeling of axons in three-dimensional electron microscopy data for long-range connectomics.eLife · 2018Article
- The Three-Dimensional Microenvironment of the Mitral Valve: Insights into the Effects of Physiological Loads.Cellular and molecular bioengineering · 2018Article
- Precise and economic FIB/SEM for CLEM: with 2 nm voxels through mitosis.Histochemistry and cell biology · 2018Article
- Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding.Frontiers in neuroanatomy · 2018Article
- Visualization of HIV T Cell Virological Synapses and Virus-Containing Compartments by Three-Dimensional Correlative Light and Electron Microscopy.Journal of virology · 2017Article
- 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy.Journal of cell science · 2017Article
- Feature Adaptive Sampling for Scanning Electron Microscopy.Scientific reports · 2016Article
- Macropinosomes are Key Players in Early Shigella Invasion and Vacuolar Escape in Epithelial Cells.PLoS pathogens · 2016Article
- Focused ion beams in biology.Nature methods · 2015Review
- FluoroNanogold: an important probe for correlative microscopy.Journal of chemical biology · 2015Review
- Preservation of protein fluorescence in embedded human dendritic cells for targeted 3D light and electron microscopy.Journal of microscopy · 2015Article
- Towards the imaging of Weibel-Palade body biogenesis by serial block face-scanning electron microscopy.Journal of microscopy · 2015Article
- Article
- Correlated light and electron microscopy: ultrastructure lights up!Nature methods · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
We report methodological advances that extend the current capabilities of ion-abrasion scanning electron microscopy (IA-SEM), also known as focused ion beam scanning electron microscopy, a newly emerging technology for high resolution imaging of large biological specimens in 3D. We establish protocols that enable the routine generation of 3D image stacks of entire plastic-embedded mammalian cells by IA-SEM at resolutions of ∼10-20nm at high contrast and with minimal artifacts from the focused ion beam. We build on these advances by describing a detailed approach for carrying out correlative live confocal microscopy and IA-SEM on the same cells. Finally, we demonstrate that by combining correlative imaging with newly developed tools for automated image processing, small 100nm-sized entities such as HIV-1 or gold beads can be localized in SEM image stacks of whole mammalian cells. We anticipate that these methods will add to the arsenal of tools available for investigating mechanisms underlying host-pathogen interactions, and more generally, the 3D subcellular architecture of mammalian cells and tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.