ArticleJournal of controlled release : official journal of the Controlled Release Society2012
Fibrin hydrogels for lentiviral gene delivery in vitro and in vivo.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The trial behind it
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Who cites it
34 citing papers in PubMed, 77 citations in OpenAlex.
- Bioengineered In Situ-Forming Hydrogels as Smart Drug Delivery Systems for Postoperative Breast Cancer Immunotherapy: From Material Innovation to Clinical Translation.Journal of functional biomaterials · 2025Review
- Development and Characterization of Adeno-Associated Virus-Loaded Coaxial Electrospun Scaffolds for Potential Viral Vector Delivery.Polymers · 2025Article
- A Novel Deer Antler-Inspired Bone Graft Triggers Rapid Bone Regeneration.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Acoustically responsive scaffolds: Unraveling release kinetics and mechanisms for sustained, steady drug delivery.Journal of controlled release : official journal of the Controlled Release Society · 2024Article
- A knock down strategy for rapid, generic, and versatile modelling of muscular dystrophies in 3D-tissue-engineered-skeletal muscle.Skeletal muscle · 2024Article
- Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells.International journal of molecular sciences · 2022Article
- Review
- Injectable BMP-2 gene-activated scaffold for the repair of cranial bone defect in mice.Stem cells translational medicine · 2020Article
- Enhanced wound healing via collagen-turnover-driven transfer of PDGF-BB gene in a murine wound model.ACS applied bio materials · 2020Article
- Spatiotemporally-controlled transgene expression in hydroxyapatite-fibrin composite scaffolds using high intensity focused ultrasound.Biomaterials · 2019Article
- Improved anti-tumor efficacy via combination of oxaliplatin and fibrin glue in colorectal cancer.Oncotarget · 2018Article
- Projection Stereolithographic Fabrication of BMP-2 Gene-activated Matrix for Bone Tissue Engineering.Scientific reports · 2017Article
- Biomaterial-Guided Gene Delivery for Musculoskeletal Tissue Repair.Tissue engineering. Part B, Reviews · 2017Review
- Laminin-111-derived peptide conjugated fibrin hydrogel restores salivary gland function.PloS one · 2017Article
- Evolving lessons on nanomaterial-coated viral vectors for local and systemic gene therapy.Nanomedicine (London, England) · 2016Review
- Use of Hydroxyapatite Doping to Enhance Responsiveness of Heat-Inducible Gene Switches to Focused Ultrasound.Ultrasound in medicine & biology · 2016Article
- Hydrogel-Based Controlled Delivery Systems for Articular Cartilage Repair.BioMed research international · 2016Article
- Surface-mediated delivery of siRNA from fibrin hydrogels for knockdown of the BMP-2 binding antagonist noggin.Acta biomaterialia · 2015Article
- Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model.Advanced healthcare materials · 2015Article
- Opportunities for multicomponent hybrid hydrogels in biomedical applications.Biomacromolecules · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Gene delivery from hydrogels represents a versatile approach for localized expression of tissue inductive factors that can promote cellular processes that lead to regeneration. Lentiviral gene therapy vectors were entrapped within fibrin hydrogels, either alone or complexed with hydroxylapatite (HA) nanoparticles. The inclusion of HA into the hydrogel led to the formation of small aggregates distributed throughout the hydrogel, with no obvious alteration of the pore structure outside the aggregates. The presence of HA slowed hydrogel degradation by collagenase and plasmin relative to fibrin alone, and also decreased the rate of cell migration. Lentivirus had similar release from the fibrin hydrogels formed with or without HA. The altered hydrogel properties suggest an interaction between the nanoparticle and fibrin, which may displace the virus from the particle leading to similar release profiles. Transgene expression by cells migrating into the hydrogel in vitro was reduced in the presence of HA, consistent with the role of cell migration on transgene expression. In vivo, lentivirus loaded fibrin hydrogels promoted localized transgene expression that increased through day 9 and decreased through day 14. For the fibrin only hydrogels, expression continued to decline after day 14. However, hydrogels with HA maintained this transgene expression level for an additional 2 weeks before declining. Immunostaining identified transgene primarily outside the fibrin-HA gel at day 9; however, at day 21, transgene expression was observed primarily within the fibrin-HA gel. The localized delivery of lentivirus provides an opportunity to enhance the bioactivity of fibrin hydrogels for a wide range of applications in regenerative medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.