ArticleCurrent biology : CB2011
Organization of the smallest eukaryotic spindle.
Article in Current biology : CB, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Power-law scaling of mitotic spindles with genome sizes across eukaryotes is driven by chromosome crowding.Nature cell biology · 2026Article
- <italic>Zea mays</italic> Meiotic Spindle Ultrastructure Reveals Kinetochore-Microtubule Interface and Embedded Membrane Components.Cytogenetic and genome research · 2025Article
- Spindle architecture constrains karyotype evolution.Nature cell biology · 2024Article
- Spindle architecture constrains karyotype in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- Three-dimensional organization of the cytoskeleton: A cryo-electron tomography perspective.Protein science : a publication of the Protein Society · 2020Review
- Investigating eukaryotic cells with cryo-ET.Molecular biology of the cell · 2020Article
- The regulation of chromosome segregation via centromere loops.Critical reviews in biochemistry and molecular biology · 2019Review
- Electron cryotomography analysis of Dam1C/DASH at the kinetochore-spindle interface in situ.The Journal of cell biology · 2019Article
- The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin.Molecular biology of the cell · 2018Article
- Natural chromatin is heterogeneous and self-associates in vitro.Molecular biology of the cell · 2018Article
- Acclimation of a low iron adapted Ostreococcus strain to iron limitation through cell biomass lowering.Scientific reports · 2017Article
- Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles.Molecular biology of the cell · 2015Article
- Scaling, selection, and evolutionary dynamics of the mitotic spindle.Current biology : CB · 2015Article
- An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.BMC genomics · 2014Article
- Dnmt1-independent CG methylation contributes to nucleosome positioning in diverse eukaryotes.Cell · 2014Article
- Article
- Review
- Exotic mitotic mechanisms.Open biology · 2012Review
- Advances in tomography: probing the molecular architecture of cells.Nature reviews. Molecular cell biology · 2012Review
- Total centromere size and genome size are strongly correlated in ten grass species.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In metazoans, plants, and fungi, the spindle checkpoint delays mitosis until each chromosome is attached to one or more of its own kinetochore microtubules (kMTs). Some unicellular eukaryotes, however, have been reported to have fewer kMTs than chromosomes [1-5]. If this is the case, it is unclear how the spindle checkpoint could be satisfied. In the vast majority of the previous studies, mitotic cells were chemically fixed at room temperature, but this does not always preserve dynamic and/or small structures like spindle MTs and kinetochores [6]. Indeed, later higher-resolution studies have reversed some earlier claims [7-11]. Here we show that in Ostreococcus tauri (the smallest eukaryote known), mitosis does involve fewer spindle microtubules than chromosomes. O. tauri cultures were enriched for mitotic cells, high-pressure frozen, and then imaged in 3D both in plastic and in a near-native ("frozen-hydrated") state through electron tomography. Mitotic cells have a distinctive intranuclear heterochromatin-free "spindle tunnel" with approximately four short and occasionally one long, incomplete (unclosed) microtubule at each end of the spindle tunnel. Because other aspects of O. tauri's spindle checkpoint seem typical, these data suggest that O. tauri's 20 chromosomes are physically linked and segregated as just one or a small number of groups.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.