ArticleBMC research notes2011
Reference genes for quantitative reverse transcription-polymerase chain reaction expression studies in wild and cultivated peanut.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
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- Towards resilience: Transcriptional insights on flavonoid biosynthesis during peanut seed maturation phases.PloS one · 2025Article
- Identification and expression profiling ofFrontiers in plant science · 2024Article
- The Stilbene Synthase Family inGenes · 2023Article
- Comparative Transcriptomic Analysis Reveals the Negative Response Mechanism of Peanut Root Morphology and Nitrate Assimilation to Nitrogen Deficiency.Plants (Basel, Switzerland) · 2023Article
- Allelic expression ofFrontiers in plant science · 2023Article
- Engineering Resistance againstPlants (Basel, Switzerland) · 2022Article
- Dissection of valine-glutamine genes and their responses to drought stress in Arachis hypogaea cv. Tifrunner.Functional & integrative genomics · 2022Article
- Comparative transcriptome analysis of pollen and anther wall reveals novel insights into the regulatory mechanisms underlying anther wall development and its dehiscence in rice.Plant cell reports · 2022Article
- Transcriptome Responses of WildPlants (Basel, Switzerland) · 2022Article
- Gene expression and DNA methylation altering lead to the high oil content in wild allotetraploid peanut (Frontiers in plant science · 2022Article
- Article
- Defining the combined stress response in wild Arachis.Scientific reports · 2021Article
- Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants.International journal of molecular sciences · 2020Article
- Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses.Scientific reports · 2020Article
- Presence of resveratrol in wild Arachis species adds new value to this overlooked genetic resource.Scientific reports · 2020Article
- Contrasting Effects of WildFrontiers in plant science · 2019Article
- Comparative root transcriptome of wild Arachis reveals NBS-LRR genes related to nematode resistance.BMC plant biology · 2018Article
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7 authors.
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Abstract
backgroundWild peanut species (Arachis spp.) are a rich source of new alleles for peanut improvement. Plant transcriptome analysis under specific experimental conditions helps the understanding of cellular processes related, for instance, to development, stress response, and crop yield. The validation of these studies has been generally accomplished by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) which requires normalization of mRNA levels among samples. This can be achieved by comparing the expression ratio between a gene of interest and a reference gene which is constitutively expressed. Nowadays there is a lack of appropriate reference genes for both wild and cultivated Arachis. The identification of such genes would allow a consistent analysis of qRT-PCR data and speed up candidate gene validation in peanut.
resultsA set of ten reference genes were analyzed in four Arachis species (A. magna; A. duranensis; A. stenosperma and A. hypogaea) subjected to biotic (root-knot nematode and leaf spot fungus) and abiotic (drought) stresses, in two distinct plant organs (roots and leaves). By the use of three programs (GeNorm, NormFinder and BestKeeper) and taking into account the entire dataset, five of these ten genes, ACT1 (actin depolymerizing factor-like protein), UBI1 (polyubiquitin), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), 60S (60S ribosomal protein L10) and UBI2 (ubiquitin/ribosomal protein S27a) emerged as top reference genes, with their stability varying in eight subsets. The former three genes were the most stable across all species, organs and treatments studied.
conclusionsThis first in-depth study of reference genes validation in wild Arachis species will allow the use of specific combinations of secure and stable reference genes in qRT-PCR assays. The use of these appropriate references characterized here should improve the accuracy and reliability of gene expression analysis in both wild and cultivated Arachis and contribute for the better understanding of gene expression in, for instance, stress tolerance/resistance mechanisms in plants.
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