ArticleInorganic chemistry2011
Reactions of the fluorescent sensor, Zinquin, with the zinc-proteome: adduct formation and ligand substitution.
Article in Inorganic chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- ZIP1 Is a Critical DNA Damage-Responsive Zinc Transporter Induced by Bleomycin via the TNFα-NF-κB Pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Optical Monitoring of Labile Zinc inside Metastatic Cells with Plasmonic Chemonanosensors.ACS omega · 2024Article
- Synthesis, DFT studies on a series of tunable quinoline derivatives.RSC advances · 2024Article
- Zinc Binding Inhibits Cellular Uptake and Antifungal Activity of Histatin-5 inACS infectious diseases · 2022Article
- Neuronal signalling of zinc: from detection and modulation to function.Open biology · 2022Review
- Human Epidermal Zinc Concentrations after Topical Application of ZnO Nanoparticles in Sunscreens.International journal of molecular sciences · 2021Article
- Sample preparation with sucrose cryoprotection dramatically alters Zn distribution in the rodent hippocampus, as revealed by elemental mapping.Journal of analytical atomic spectrometry · 2020Article
- Antimicrobial action of calprotectin that does not involve metal withholding.Metallomics : integrated biometal science · 2018Article
- Rational Design and Facile Synthesis of a Highly Tunable Quinoline-Based Fluorescent Small-Molecule Scaffold for Live Cell Imaging.Journal of the American Chemical Society · 2018Article
- Proteomic High Affinity ZnInternational journal of molecular sciences · 2017Review
- Mapping the dynamics of cortical neuroplasticity of skilled motor learning using micro X-ray fluorescence and histofluorescence imaging of zinc in the rat.Behavioural brain research · 2017Article
- Newport Green, a fluorescent sensor of weakly bound cellular Zn(2+): competition with proteome for Zn(2).Metallomics : integrated biometal science · 2016Article
- Vimentin filament organization and stress sensing depend on its single cysteine residue and zinc binding.Nature communications · 2015Article
- Impact of Cadmium on Intracellular Zinc Levels in HepG2 Cells: Quantitative Evaluations and Molecular Effects.BioMed research international · 2015Article
- Laminar-specific distribution of zinc: evidence for presence of layer IV in forelimb motor cortex in the rat.NeuroImage · 2014Article
- Elemental and chemically specific X-ray fluorescence imaging of biological systems.Chemical reviews · 2014Review
- Toxic metal proteomics: reaction of the mammalian zinc proteome with Cd²⁺.Journal of inorganic biochemistry · 2014Article
- Native SDS-PAGE: high resolution electrophoretic separation of proteins with retention of native properties including bound metal ions.Metallomics : integrated biometal science · 2014Article
- Fluorescent sensors for measuring metal ions in living systems.Chemical reviews · 2014Review
- Zn(II)-coordination modulated ligand photophysical processes - the development of fluorescent indicators for imaging biological Zn(II) ions.RSC advances · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Zinquin (ZQ) is a commonly used sensor for cellular Zn(2+) status. It has been assumed that it measures accessible Zn(2+) concentrations in the nanomolar range. Instead, this report shows a consistent pattern across seven mammalian cell and tissue types that ZQ reacts with micromolar concentrations of Zn(2+) bound as Zn-proteins. The predominant class of products were ZQ-Zn-protein adducts that were characterized in vivo and in vitro by a fluorescence emission spectrum centered at about 470 nm, by their migration over Sephadex G-75 as protein not low molecular weight species, by the exclusion of reaction with lipid vesicles, and by their large aggregate concentration. In addition, variable, minor formation of Zn(ZQ)(2) with a fluorescence band at about 490 nm was observed in vivo in each case. Because incubation of isolated Zn-proteome with ZQ also generated similar amounts of Zn(ZQ)(2), it was concluded that this species had formed through direct ligand substitution in which ZQ had successfully competed for protein-bound Zn(2+). Parallel studies with the model Zn-proteins, alcohol dehydrogenase (ADH), and alkaline phosphatase (AP) revealed a similar picture of reactivity: ZQ(ACID) (Zinquin acid, (2-methyl-8-p-toluenesulfonamido-6-quinolyloxy)acetate)) able to bind to one Zn(2+) and extract the other in Zn(2)-ADH, whereas it removed one Zn(2+) from Zn(2)-AP and did not bind to the other. Zinquin ethyl ester (ethyl(2-methyl-8-p-toluenesulfonamido-6-quinolyloxy)acetate); ZQ(EE)) bound to both proteins without sequestering Zn(2+) from either one. In contrast to a closely related sensor, 6-methoxy-8-p-toluenesulfonamido-quinoline (TSQ), neither ZQ(ACID) nor ZQ(EE) associated with Zn-carbonic anhydrase. A survey of reactivity of these sensors with partially fractionated Zn-proteome confirmed that ZQ and TSQ bind to distinct, overlapping subsets of the Zn-proteome.
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