ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2011
Induction of DNA damage signaling by oxidative stress in relation to DNA replication as detected using "click chemistry".
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- The role of oxidative stress in 63 T-induced cytotoxicity against human lung cancer and normal lung fibroblast cell lines.Investigational new drugs · 2019Article
- Adult Cardiomyocyte Cell Cycle Detour: Off-ramp to Quiescent Destinations.Trends in endocrinology and metabolism: TEM · 2019Review
- High Basal Levels of γH2AX in Human Induced Pluripotent Stem Cells Are Linked to Replication-Associated DNA Damage and Repair.Stem cells (Dayton, Ohio) · 2018Article
- High Dose Ascorbate Causes Both Genotoxic and Metabolic Stress in Glioma Cells.Antioxidants (Basel, Switzerland) · 2017Article
- Tuberatolide B Suppresses Cancer Progression by Promoting ROS-Mediated Inhibition of STAT3 Signaling.Marine drugs · 2017Article
- Dr Jekyll and Mr Hyde: a strange case of 5-ethynyl-2'-deoxyuridine and 5-ethynyl-2'-deoxycytidine.Open biology · 2016Article
- Molecular and Cellular Effects of Hydrogen Peroxide on Human Lung Cancer Cells: Potential Therapeutic Implications.Oxidative medicine and cellular longevity · 2016Review
- DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblasts.BMC molecular biology · 2015Article
- Article
- DNA damage signaling, impairment of cell cycle progression, and apoptosis triggered by 5-ethynyl-2'-deoxyuridine incorporated into DNA.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- New insights into cell cycle and DNA damage response machineries through high-resolution AMICO quantitative imaging cytometry.Cell proliferation · 2013Article
- Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Analysis of spatial correlations between patterns of DNA damage response and DNA replication in nuclei of cells subjected to replication stress or oxidative damage.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Real-time cell viability assays using a new anthracycline derivative DRAQ7®.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Laser scanning cytometry: principles and applications-an update.Methods in molecular biology (Clifton, N.J.) · 2013Review
- The canonical NF-κB pathway differentially protects normal and human tumor cells from ROS-induced DNA damage.Cellular signalling · 2012Article
- Article
- Targeting the cancer cell cycle by cold atmospheric plasma.Scientific reports · 2012Article
- Relationship of DNA damage signaling to DNA replication following treatment with DNA topoisomerase inhibitors camptothecin/topotecan, mitoxantrone, or etoposide.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2012Article
- DNA damage signaling assessed in individual cells in relation to the cell cycle phase and induction of apoptosis.Critical reviews in clinical laboratory sciencesReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Induction of DNA damage by oxidants such as H(2) O(2) activates the complex network of DNA damage response (DDR) pathways present in cells to initiate DNA repair, halt cell cycle progression, and prepare an apoptotic reaction. We have previously reported that activation of Ataxia Telangiectasia Mutated protein kinase (ATM) and induction of γH2AX are among the early events of the DDR induced by exposure of cells to H(2) O(2) , and in human pulmonary carcinoma A549 cells, both events were expressed predominantly during S-phase. This study was designed to further explore a correlation between these events and DNA replication. Toward this end, we utilized 5-ethynyl-2'deoxyuridine (EdU) and the "click chemistry" approach to label DNA during replication, followed by exposure of A549 cells to H(2) O(2) . Multiparameter laser scanning cytometric analysis of these cells made it possible to identify DNA replicating cells and directly correlate H(2) O(2) -induced ATM activation and induction of γH2AX with DNA replication on a cell by cell basis. After pulse-labeling with EdU and exposure to H(2) O(2) , confocal microscopy was also used to examine the localization of DNA replication sites ("replication factories") versus the H2AX phosphorylation sites (γH2AX foci) in nuclear chromatin in an attempt to observe the absence or presence of colocalization. The data indicate a close association between DNA replication and H2AX phosphorylation in A549 cells, suggesting that these DNA damage response events may be triggered by stalled replication forks and perhaps also by induction of DNA double-strand breaks at the primary DNA lesions induced by H(2) O(2) .
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.