ArticleLipids2011
Electrospray ionization tandem mass spectrometry of sodiated adducts of cholesteryl esters.
Article in Lipids, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Oxidized Lipoproteins Promote Resistance to Cancer Immunotherapy Independent of Patient Obesity.Cancer immunology research · 2021Trial
- Reprogramming lipid metabolism prevents effector T cell senescence and enhances tumor immunotherapy.Science translational medicine · 2021Article
- Mass Spectrometry-Based Shotgun Lipidomics for Cancer Research.Advances in experimental medicine and biology · 2021Article
- Tutorial on lipidomics.Analytica chimica acta · 2019Review
- Transfer of Functional Cargo in Exomeres.Cell reports · 2019Article
- Article
- Uncovering Structural Diversity of Unsaturated Fatty Acyls in Cholesteryl Esters via Photochemical Reaction and Tandem Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Lpcat3-dependent production of arachidonoyl phospholipids is a key determinant of triglyceride secretion.eLife · 2015Article
- Polarized THG microscopy identifies compositionally different lipid droplets in mammalian cells.Biophysical journal · 2014Article
- Article
- Article
- Differential regulation of ABCA1 and macrophage cholesterol efflux by elaidic and oleic acids.Lipids · 2013Article
- Cholesterol efflux analyses using stable isotopes and mass spectrometry.Analytical biochemistry · 2013Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Cholesteryl esters (CE) are important lipid storage molecules. The present study demonstrates that sodiated adducts of CE molecular species form positive ions that can be detected in both survey scan mode as well as by exploiting class-specific fragmentation in MS/MS scan modes. A common neutral loss for CE is the loss of cholestane (NL 368.5), which can be used to specifically quantify tissue CE molecular species. Using this MS/MS technique, CE molecular species were quantified in mouse monocyte-derived macrophages (J774 cells) incubated with either linoleic (18:2) or arachidonic acid (20:4). These studies revealed that arachidonic acid was not only incorporated into the CE pool, but also was elongated resulting in the accumulation of 22:4 and 24:4 CE molecular species in macrophages. Additionally, this technique was used to quantify CE molecular species present in crude lipid extracts from plasma of female mice fed a Western diet, which led to an enrichment in CE molecular species containing monounsaturated fatty acids compared to female mice fed a normal chow diet. Last, NL 368.5 spectra revealed the oxidation of the aliphatic fatty acid residues of CE molecular species containing polyunsaturated fatty acids. Taken together, these studies demonstrate the utility of using sodiated adducts of CE in conjunction with direct infusion electrospray ionization tandem mass spectrometry to rapidly quantify CE molecular species in biological samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.