ArticleAmerican journal of translational research2011
HaloTag: a novel reporter gene for positron emission tomography.
Article in American journal of translational research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Click biology highlights the opportunities from reliable biological reactions.Nature chemical biology · 2025Review
- Recent biomedical advances enabled by HaloTag technology.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2022Article
- Quantum dots in biomedical applications.Acta biomaterialia · 2019Review
- A Fluorescent, [ACS chemical biology · 2019Article
- Targeted delivery of gold nanoparticle contrast agents for reporting gene detection by magnetic resonance imaging.Chemical communications (Cambridge, England) · 2016Article
- HaloTag technology: a versatile platform for biomedical applications.Bioconjugate chemistry · 2015Review
- Genetic targeting of a small fluorescent zinc indicator to cell surface for monitoring zinc secretion.ACS chemical biology · 2015Article
- Imaging tumor angiogenesis in breast cancer experimental lung metastasis with positron emission tomography, near-infrared fluorescence, and bioluminescence.Angiogenesis · 2013Article
- Application of HaloTag technology to expression and purification of cannabinoid receptor CB2.Protein expression and purification · 2013Article
- PET imaging of CD105/endoglin expression with a ⁶¹/⁶⁴Cu-labeled Fab antibody fragment.European journal of nuclear medicine and molecular imaging · 2013Article
- HaloTag as a reporter gene: positron emission tomography imaging with (64)Cu-labeled second generation HaloTag ligands.American journal of translational research · 2013Article
- Positron emission tomography imaging of vascular endothelial growth factor receptor expression with (61)Cu-labeled lysine-tagged VEGF121.Molecular pharmaceutics · 2012Article
- Immuno-PET of tissue factor in pancreatic cancer.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2012Article
- Multifunctional unimolecular micelles for cancer-targeted drug delivery and positron emission tomography imaging.Biomaterials · 2012Article
- In vivo targeting and imaging of tumor vasculature with radiolabeled, antibody-conjugated nanographene.ACS nano · 2012Article
- HaloTag, a Platform Technology for Protein Analysis.Current chemical genomics · 2012Article
- HaloTag® Platform: From Proteomics to Cellular Analysis and Animal Imaging.Current chemical genomics · 2012Article
- Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.Current chemical genomics · 2012Article
- Positron emission tomography imaging of CD105 expression with a 64Cu-labeled monoclonal antibody: NOTA is superior to DOTA.PloS one · 2011Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Among the many molecular imaging techniques, reporter gene imaging has been a dynamic area of research. The HaloTag protein is a modified haloalkane dehalogenase which was designed to covalently bind to synthetic ligands (i.e. the HaloTag ligands [HTL]). Covalent bond formation between the HaloTag protein and the chloroal-kane within the HTL occurs rapidly under physiological conditions, which is highly specific and essentially irreversible. Over the years, HaloTag technology has been investigated for various applications such as in vitro/in vivo imaging, protein purification/trafficking, high-throughput assays, among others. The goal of this study is to explore the use of the HaloTag protein as a novel reporter gene for positron emission tomography (PET) imaging. By attaching a HaloTag -reactive chloroalkane to 1, 4, 7-triazacyclononane-N, N', N"-triacetic acid (NOTA) through hydrophilic linkers, the resulting NOTA-conjugated HTLs were labeled with (64)Cu and tested for PET imaging in living mice bearing 4T1-HaloTag-ECS tumors, which stably express the HaloTag protein on the cell surface. Significantly higher uptake of (64)Cu-NOTA-HTL-S (which contains a short hydrophilic linker) in the 4T1-HaloTag-ECS than the non-HaloTag-expressing 4T1 tumors was observed, which demonstrated the HaloTag specificity of (64)Cu-NOTA-HTL-S and warranted future investigation of the HaloTag protein as a PET reporter gene.
Indexed as
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21904659PMC3158741What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.