ArticlePloS one2011
Comparative proteomic analysis of Methanothermobacter themautotrophicus ΔH in pure culture and in co-culture with a butyrate-oxidizing bacterium.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 31 citations in OpenAlex.
- Proteome-informed interrogation of a methanogenic archaeon links stress response mechanisms with microbial performance and robustness during biomethanation.Engineering microbiology · 2026Article
- Metagenomic and cultivation-based description of a syntrophic butyrate-oxidizing bacterium from a thermophilic and high-ammonia biogas process.BMC microbiology · 2026Article
- Methyl coenzyme M reductase as a target for inhibition of methanogenesis in ruminants: challenges and opportunities.Frontiers in microbiology · 2025Review
- Article
- Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode.Archaea (Vancouver, B.C.) · 2021Article
- An iron corrosion-assisted HScientific reports · 2020Article
- Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from HMicrobiologyOpen · 2019Article
- Growth Kinetics, Carbon Isotope Fractionation, and Gene Expression in the HyperthermophileApplied and environmental microbiology · 2019Article
- Alternative Pathways of Acetogenic Ethanol and Methanol Degradation in the Thermophilic AnaerobeFrontiers in microbiology · 2019Article
- Energy-Conserving Enzyme Systems Active During Syntrophic Acetate Oxidation in the Thermophilic BacteriumFrontiers in microbiology · 2019Article
- Label-Free Proteomics of a Defined, Binary Co-culture Reveals Diversity of Competitive Responses Between Members of a Model Soil Microbial System.Microbial ecology · 2018Article
- Article
- Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture.Scientific reports · 2018Article
- Concerted Metabolic Shifts Give New Insights Into the Syntrophic Mechanism Between Propionate-FermentingFrontiers in microbiology · 2018Article
- Genomic composition and dynamics among Methanomicrobiales predict adaptation to contrasting environments.The ISME journal · 2017Article
- Cultural, Transcriptomic, and Proteomic Analyses of Water-Stressed Cells of Actinobacterial Strains Isolated from Compost: Ecological Implications in the Fed-Batch Composting Process.Microbes and environments · 2016Article
- Methane yield phenotypes linked to differential gene expression in the sheep rumen microbiome.Genome research · 2014Article
- Response of a rice paddy soil methanogen to syntrophic growth as revealed by transcriptional analyses.Applied and environmental microbiology · 2014Article
- Co-culture systems and technologies: taking synthetic biology to the next level.Journal of the Royal Society, Interface · 2014Review
- The challenges of studying the anaerobic microbial world.Microbes and environments · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the physiological basis of methanogenic archaea living on interspecies H(2) transfer, the protein expression of a hydrogenotrophic methanogen, Methanothermobacter thermautotrophicus strain ΔH, was investigated in both pure culture and syntrophic coculture with an anaerobic butyrate oxidizer Syntrophothermus lipocalidus strain TGB-C1 as an H(2) supplier. Comparative proteomic analysis showed that global protein expression of methanogen cells in the model coculture was substantially different from that of pure cultured cells. In brief, in syntrophic coculture, although methanogenesis-driven energy generation appeared to be maintained by shifting the pathway to the alternative methyl coenzyme M reductase isozyme I and cofactor F(420)-dependent process, the machinery proteins involved in carbon fixation, amino acid synthesis, and RNA/DNA metabolisms tended to be down-regulated, indicating restrained cell growth rather than vigorous proliferation. In addition, our proteome analysis revealed that α subunits of proteasome were differentially acetylated between the two culture conditions. Since the relevant modification has been suspected to regulate proteolytic activity of the proteasome, the global protein turnover rate could be controlled under syntrophic growth conditions. To our knowledge, the present study is the first report on N-acetylation of proteasome subunits in methanogenic archaea. These results clearly indicated that physiological adaptation of hydrogenotrophic methanogens to syntrophic growth is more complicated than that of hitherto proposed.
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