ArticleActa crystallographica. Section F, Structural biology and crystallization communications2011
Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline.
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- SAS: Split antibiotic selection for identifying chaperones that improve protein solubility.Heliyon · 2024Article
- Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology.Frontiers in molecular biosciences · 2021Review
- Biochemical characterization of a glycosyltransferase Gtf3 from3 Biotech · 2020Article
- Autoantibodies to killer cell immunoglobulin-like receptor 3DL1 in patients with systemic lupus erythematosus.Clinical and experimental immunology · 2019Article
- RNAi-Based Biocontrol of Wheat Nematodes Using Natural Poly-Component Biostimulants.Frontiers in plant science · 2019Article
- Amphipols for each season.The Journal of membrane biology · 2014Review
- Development of a full-length human protein production pipeline.Protein science : a publication of the Protein Society · 2014Article
- Development of a functional antibody by using a green fluorescent protein frame as the template.Applied and environmental microbiology · 2014Article
- Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp.Frontiers in cellular and infection microbiology · 2013Article
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Authors and funding
7 authors.
Funding
Abstract
Recombinant expression of proteins of interest in Escherichia coli is an important tool in the determination of protein structure. However, lack of expression and insolubility remain significant challenges to the expression and crystallization of these proteins. The SSGCID program uses a wheat germ cell-free expression system as a rescue pathway for proteins that are either not expressed or insoluble when produced in E. coli. Testing indicates that the system is a valuable tool for these protein targets. Further increases in solubility were obtained by the addition of the NVoy polymer reagent to the reaction mixture. These data indicate that this eukaryotic cell-free expression system has a high success rate and that the addition of specific reagents can increase the yield of soluble protein.
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