Evidence map›Paper›PMID 21904044›Full record

ArticleActa crystallographica. Section F, Structural biology and crystallization communications2011

Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics.

Jeff Christensen, Cory J Gerdts, Mathew C Clifton, Lance Stewart

Open access · hybridAbstract read
In one paragraph

Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Inhibitor-bound complexes of dihydrofolate reductase-thymidylate synthase from Babesia bovis.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jeff ChristensenEmerald BioStructures, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA.
Cory J Gerdts
Mathew C Clifton
Lance Stewart

Funding

Sub-project 5U54GM074961 · NIGMS · EMERALD BIOSTRUCTURES · PI RUTH, RONALD D. · 2005 to 2009
$19.3M
NIAID NIH HHS HHSN272200700057CNIGMS NIH HHS U54 GM074961
6 · The paper itself

Abstract

The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method.

Indexed as

CrystallizationCrystallography, X-RayMicrofluidicsProteinsProteins

Identifiers

PMID21904044
PMCPMC3169396
OpenAlexW2134269930

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.