ArticleActa crystallographica. Section F, Structural biology and crystallization communications2011
Gene design, cloning and protein-expression methods for high-value targets at the Seattle Structural Genomics Center for Infectious Disease.
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Crystal structure of a short-chain dehydrogenase/reductase from Burkholderia phymatum in complex with NAD.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Crystal structure of chorismate mutase from Burkholderia phymatum.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Mycobacterium tuberculosis Rv3651 is a triple sensor-domain protein.Protein science : a publication of the Protein Society · 2018Article
- Multi-target parallel processing approach for gene-to-structure determination of the influenza polymerase PB2 subunit.Journal of visualized experiments : JoVE · 2013Article
- Structure and function of a cyanophage-encoded peptide deformylase.The ISME journal · 2013Article
- Artificial neural networks trained to detect viral and phage structural proteins.PLoS computational biology · 2012Article
- Gene Composer in a structural genomics environment.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Any structural genomics endeavor, particularly ambitious ones such as the NIAID-funded Seattle Structural Genomics Center for Infectious Disease (SSGCID) and Center for Structural Genomics of Infectious Disease (CSGID), face technical challenges at all points of the production pipeline. One salvage strategy employed by SSGCID is combined gene engineering and structure-guided construct design to overcome challenges at the levels of protein expression and protein crystallization. Multiple constructs of each target are cloned in parallel using Polymerase Incomplete Primer Extension cloning and small-scale expressions of these are rapidly analyzed by capillary electrophoresis. Using the methods reported here, which have proven particularly useful for high-value targets, otherwise intractable targets can be resolved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.