ArticleActa crystallographica. Section D, Biological crystallography2011
Dark progression reveals slow timescales for radiation damage between T = 180 and 240 K.
Article in Acta crystallographica. Section D, Biological crystallography, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Damage-limited resolution for X-ray and electron microscopy of organic specimens.Acta crystallographica. Section D, Structural biology · 2026Article
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- Obtaining anomalous and ensemble information from protein crystals from 220 K up to physiological temperatures.Acta crystallographica. Section D, Structural biology · 2023Article
- Refinement of multiconformer ensemble models from multi-temperature X-ray diffraction data.Methods in enzymology · 2023Article
- Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization.Acta crystallographica. Section D, Structural biology · 2023Article
- Evaluating the impact of X-ray damage on conformational heterogeneity in room-temperature (277 K) and cryo-cooled protein crystals.Acta crystallographica. Section D, Structural biology · 2022Article
- Instrumentation and experimental procedures for robust collection of X-ray diffraction data from protein crystals across physiological temperatures.Journal of applied crystallography · 2020Article
- Radiation-driven rotational motion of nanoparticles.Journal of synchrotron radiation · 2018Article
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- Temperature-dependent radiation sensitivity and order of 70S ribosome crystals.Acta crystallographica. Section D, Biological crystallography · 2014Article
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- Exploiting fast detectors to enter a new dimension in room-temperature crystallography.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Matching X-ray beam and detector properties to protein crystals of different perfection.Journal of synchrotron radiation · 2014Article
- Kinetic modeling of the X-ray-induced damage to a metalloprotein.The journal of physical chemistry. B · 2013Article
- Global radiation damage: temperature dependence, time dependence and how to outrun it.Journal of synchrotron radiation · 2013Article
- A survey of global radiation damage to 15 different protein crystal types at room temperature: a new decay model.Journal of synchrotron radiation · 2013Article
- Micro-crystallography comes of age.Current opinion in structural biology · 2012Review
- Spatial distribution of radiation damage to crystalline proteins at 25-300 K.Acta crystallographica. Section D, Biological crystallography · 2012Article
- Outrunning free radicals in room-temperature macromolecular crystallography.Acta crystallographica. Section D, Biological crystallography · 2012Article
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4 authors.
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Abstract
Can radiation damage to protein crystals be `outrun' by collecting a structural data set before damage is manifested? Recent experiments using ultra-intense pulses from a free-electron laser show that the answer is yes. Here, evidence is presented that significant reductions in global damage at temperatures above 200 K may be possible using conventional X-ray sources and current or soon-to-be available detectors. Specifically, `dark progression' (an increase in damage with time after the X-rays have been turned off) was observed at temperatures between 180 and 240 K and on timescales from 200 to 1200 s. This allowed estimation of the temperature-dependent timescale for damage. The rate of dark progression is consistent with an Arrhenius law with an activation energy of 14 kJ mol(-1). This is comparable to the activation energy for the solvent-coupled diffusive damage processes responsible for the rapid increase in radiation sensitivity as crystals are warmed above the glass transition near 200 K. Analysis suggests that at T = 300 K data-collection times of the order of 1 s (and longer at lower temperatures) may allow significant reductions in global radiation damage, facilitating structure solution on crystals with liquid solvent. No dark progression was observed below T = 180 K, indicating that no important damage process is slowed through this timescale window in this temperature range.
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