ArticleThe Journal of biological chemistry2011
Two-pore channels form homo- and heterodimers.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- The protein interaction networks of mucolipins and two-pore channels.Biochimica et biophysica acta. Molecular cell research · 2019Pooled it
- Endosomal TPC1-mediated CaProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Endolysosomal two-pore channels regulate a conserved entry pathway for enteroviruses.The Journal of general virology · 2026Article
- Review
- TPC Functions in the Immune System.Handbook of experimental pharmacology · 2023Article
- NAADP-Mediated CaHandbook of experimental pharmacology · 2023Article
- Targeting the two-pore channel 2 in cancer progression and metastasis.Exploration of targeted anti-tumor therapy · 2022Review
- A rare missense variant in the ATP2C2 gene is associated with language impairment and related measures.Human molecular genetics · 2021Article
- NAADP-regulated two-pore channels drive phagocytosis through endo-lysosomal CaThe EMBO journal · 2020Article
- NAADP Receptors.Cold Spring Harbor perspectives in biology · 2019Review
- Endolysosomal CaCancers · 2018Review
- Hippocampal mGluR1-dependent long-term potentiation requires NAADP-mediated acidic store CaScience signaling · 2018Article
- Two-pore channels and disease.Biochimica et biophysica acta. Molecular cell research · 2018Review
- On the structure and mechanism of two-pore channels.The FEBS journal · 2018Article
- NAADP-evoked CaCell calcium · 2017Article
- The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes.Scientific reports · 2017Article
- Two-pore channels (TPCs): Novel voltage-gated ion channels with pleiotropic functions.Channels (Austin, Tex.) · 2017Review
- Isolated pores dissected from human two-pore channel 2 are functional.Scientific reports · 2016Article
- The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix.Cellular and molecular life sciences : CMLS · 2016Article
- Ebolavirus Glycoprotein Directs Fusion through NPC1+ Endolysosomes.Journal of virology · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Two-pore channels (TPCs) have been recently identified as NAADP-regulated Ca(2+) release channels, which are localized on the endolysosomal system. TPCs have a 12-transmembrane domain (TMD) structure and are evolutionary intermediates between the 24-TMD α-subunits of Na(+) or Ca(2+) channels and the transient receptor potential channel superfamily, which have six TMDs in a single subunit and form tetramers with 24 TMDs as active channels. Based on this relationship, it is predicted that TPCs dimerize to form functional channels, but the dimerization of human TPCs has so far not been studied. Using co-immunoprecipitation studies and a mass spectroscopic analysis of the immunocomplex, we show the presence of homo- and heteromeric complexes for human TPC1 and TPC2. Despite their largely distinct localization, we identified a discrete number of endosomes that coexpressed TPC1 and TPC2. Homo- and heteromerization were confirmed by a FRET study, showing that both proteins interacted in a rotational (N- to C-terminal/head-to-tail) symmetry. This is the first report describing the presence of homomultimeric TPC1 channels and the first study showing that TPCs are capable of forming heteromers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.