ArticleMethods (San Diego, Calif.)2011
Characterization of lipid matrices for membrane protein crystallization by high-throughput small angle X-ray scattering.
Article in Methods (San Diego, Calif.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Collapse of Lipid Membranes into Distended Lipidic Cubic Phases at High Solvent Levels, Membrane Remodelling, and Self-Repair.Journal of the American Chemical Society · 2025Article
- Crystallization of Ethylene Plant Hormone Receptor-Screening for Structure.Biomolecules · 2024Article
- 3D-printed SAXS chamber for controlled in situ dialysis and optical characterization.Journal of synchrotron radiation · 2022Article
- Anomalous Diffusion Characterization by Fourier Transform-FRAP with Patterned Illumination.Biophysical journal · 2020Article
- Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.Current opinion in structural biology · 2019Review
- Chemically Stable Lipids for Membrane Protein Crystallization.Crystal growth & design · 2017Article
- Article
- High-resolution structure of a membrane protein transferred from amphipol to a lipidic mesophase.The Journal of membrane biology · 2014Article
- Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography.Nature protocols · 2014Article
- Effects of detergent β-octylglucoside and phosphate salt solutions on phase behavior of monoolein mesophases.Biophysical journal · 2013Article
- An X-ray transparent microfluidic platform for screening of the phase behavior of lipidic mesophases.The Analyst · 2013Article
- Using macromolecular-crystallography beamline and microfluidic platform for small-angle diffraction studies of lipidic matrices for membrane-protein crystallization.Journal of physics. Conference series · 2013Article
- Macrophages sequester clofazimine in an intracellular liquid crystal-like supramolecular organization.PloS one · 2012Article
- Lipidic cubic phase technologies for membrane protein structural studies.Current opinion in structural biology · 2011Review
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Authors and funding
6 authors.
Funding
Abstract
The lipidic cubic phase (LCP) has repeatedly proven to serve as a successful membrane-mimetic matrix for a variety of difficult-to-crystallize membrane proteins. While monoolein has been the predominant lipid of choice, there is a growing need for the characterization and use of other LCP host lipids, allowing exploration of a range of structural parameters such as bilayer thickness and curvature for optimal insertion, stability and crystallogenesis of membrane proteins. Here, we describe the development of a high-throughput (HT) pipeline to employ small angle X-ray scattering (SAXS) - the most direct technique to identify lipid mesophases and measure their structural parameters - to interrogate rapidly a large number of lipid samples under a variety of conditions, similar to those encountered during crystallization. Leveraging the identical setup format for LCP crystallization trials, this method allows the quickly assessment of lipid matrices for their utility in membrane protein crystallization, and could inform the tailoring of lipid and precipitant conditions to overcome specific crystallization challenges. As proof of concept, we present HT LCP-SAXS analysis of lipid samples made of monoolein with and without cholesterol, and of monovaccenin, equilibrated with solutions used for crystallization trials and LCP fluorescence recovery after photobleaching (FRAP) experiments.
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