ArticleNeurotoxicology and teratology
In vivo imaging and quantitative analysis of changes in axon length using transgenic zebrafish embryos.
Article in Neurotoxicology and teratology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
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- Progress and promise of alternative animal and non-animal methods in biomedical research.Archives of toxicology · 2023Review
- Neuropathy-associated Fars2 deficiency affects neuronal development and potentiates neuronal apoptosis by impairing mitochondrial function.Cell & bioscience · 2022Article
- A versatile, automated and high-throughput drug screening platform for zebrafish embryos.Biology open · 2021Article
- Pristine graphene and graphene oxide induce multi-organ defects in zebrafish (Danio rerio) larvae/juvenile: an in vivo study.Environmental science and pollution research international · 2021Article
- miR-31 promotes neural stem cell proliferation and restores motor function after spinal cord injury.Experimental biology and medicine (Maywood, N.J.) · 2021Article
- Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand-Receptor Interaction Signaling Pathway in Zebrafish Embryos.International journal of nanomedicine · 2020Article
- Generation of a Triple-Transgenic Zebrafish Line for Assessment of Developmental Neurotoxicity during Neuronal Differentiation.Pharmaceuticals (Basel, Switzerland) · 2019Article
- Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System.Journal of visualized experiments : JoVE · 2018Article
- Zebrafish: A Model for the Study of Toxicants Affecting Muscle Development and Function.International journal of molecular sciences · 2016Review
- Generation of Tg(cyp1a:gfp) Transgenic Zebrafish for Development of a Convenient and Sensitive In Vivo Assay for Aryl Hydrocarbon Receptor Activity.Marine biotechnology (New York, N.Y.) · 2015Article
- Developmental toxicity assay using high content screening of zebrafish embryos.Journal of applied toxicology : JAT · 2015Article
- Zebrafish: A marvel of high-throughput biology for 21Current environmental health reports · 2014Article
- Development of a convenient in vivo hepatotoxin assay using a transgenic zebrafish line with liver-specific DsRed expression.PloS one · 2014Article
- Automated processing of zebrafish imaging data: a survey.Zebrafish · 2013Review
- Primary neuron culture for nerve growth and axon guidance studies in zebrafish (Danio rerio).PloS one · 2013Article
- Fluorescent transgenic zebrafish Tg(nkx2.2a:mEGFP) provides a highly sensitive monitoring tool for neurotoxins.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We describe an imaging procedure to measure axon length in zebrafish embryos in vivo. Automated fluorescent image acquisition was performed with the ImageXpress Micro high content screening reader and further analysis of axon lengths was performed on archived images using AcuityXpress software. We utilized the Neurite Outgrowth Application module with a customized protocol (journal) to measure the axons. Since higher doses of ethanol (2-2.5%, v/v) have been shown to deform motor neurons and axons during development, here we used ethanol to treat transgenic [hb9:GFP (green fluorescent protein)] zebrafish embryos at 28 hpf (hours post-fertilization). These embryos express GFP in the motor neurons and their axons. Embryos after ethanol treatment were arrayed in 384-well plates for automated fluorescent image acquisition in vivo. Average axon lengths of high dose ethanol-treated embryos were significantly lower than the control. Another experiment showed that there was no significant difference in the axon lengths between the embryos grown for 24h at 22°C and 28.5°C. These test experiments demonstrate that using axon development as an end-point, compound screening can be performed in a time-efficient manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.