ArticleJournal of immunological methods2011
Development and validation of a standardized ELISA for the detection of soluble Fc-epsilon-RI in human serum.
Article in Journal of immunological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.Analytical chemistry · 2026Article
- A newly developed rapid K39-based LeishmaCHECK Ab ELISA for high-throughput serological screening of canine leishmaniasis.Current research in parasitology & vector-borne diseases · 2026Article
- Development of a New Indirect ELISA Test for the Detection of Anti-Feline Coronavirus Antibodies in Cats.Veterinary sciences · 2025Article
- A New Rapid Indirect ELISA Test for Serological Diagnosis of Feline Immunodeficiency.Veterinary sciences · 2025Article
- Evaluation of a newly developed rapid ELISA to detect anti-Ehrlichia canis antibodies in dogs.Parasite (Paris, France) · 2025Article
- Feline Leukemia Virus in Cats: A Novel Rapid ELISA Assay for p27 Antigen Detection.Veterinary medicine international · 2025Article
- Development of an Enzyme-Linked Immunosorbent Assay (ELISA) as a tool to detect NS1 of dengue virus serotype 2 in femaleHeliyon · 2024Article
- Engineering a versatile and retrievable cell macroencapsulation device for the delivery of therapeutic proteins.iScience · 2023Article
- Article
- The relationship between released soluble FceRI-alpha and its cell surface density on human basophils.PloS one · 2021Article
- Soluble FcɛRI: A biomarker for IgE-mediated diseases.Allergy · 2019Article
- The soluble isoform of human FcɛRI is an endogenous inhibitor of IgE-mediated mast cell responses.Allergy · 2019Article
- Elevated levels of leukotriene C4 synthase mRNA distinguish a subpopulation of eosinophilic oesophagitis patients.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2013Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The aim of this study was to develop a standardized enzyme-linked immunosorbent assay (ELISA) for detection of human soluble Fc-epsilon-RI (sFcεRI), a serum isoform of the high affinity IgE receptor. A recombinant version of sFcεRI was produced in baculovirus and used as standard. ELISA plates were coated with anti-mouse IgG followed by incubation with the monoclonal capture antibody CRA1. This FcεRI-alpha-specific antibody binds to the stalk region of the protein and does not inhibit IgE-binding. After incubation with standards or serum samples, plates were incubated with chimeric IgE followed by detection with horseradish peroxidase conjugated anti-human IgE. Enzymatic activity was visualized with (3,3',5,5')-tetramethylbenzidine. Specificity was demonstrated by omission of capture or detection reagents. Units (U) of detection were established and the dynamic range of the assay was defined as 10-640 U/ml for a 1/5 serum dilution. Parameters of linearity (R(2)>0.999), matrix interference test (recovery of 70-110%), intra-assay variability (coefficient of variation (CV) <20%) and inter-assay variability (CV <20%) met acceptance criteria for immunoassay validation. Correlation analysis of serum units of sFcεRI measured with the new ELISA and serum IgE levels confirmed earlier published data describing a weak correlation of the two parameters in patients with elevated serum IgE while no correlation in patients with normal serum IgE or the total patient group was found. In summary, we established and validated a standardized ELISA for the detection of sFcεRI. This novel method now allows for comparative analysis of sFcεRI levels in health and disease.
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