ArticleTissue engineering. Part A2012
Improved mesenchymal stem cells attachment and in vitro cartilage tissue formation on chitosan-modified poly(L-lactide-co-epsilon-caprolactone) scaffold.
Article in Tissue engineering. Part A, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 87 citations in OpenAlex.
- Application of PLCL as a biodegradable polymer in biomedical engineering.Journal of materials science. Materials in medicine · 2026Review
- Biosponge-Encased Placental Stem Cells for Volumetric Muscle Loss Repair.Advances in wound care · 2025Article
- Synergistic effects of 3D chitosan-based hybrid scaffolds and mesenchymal stem cells in orthopaedic tissue engineering.IET nanobiotechnology · 2023Review
- Chitosan coatings with distinct innate immune bioactivities differentially stimulate angiogenesis, osteogenesis and chondrogenesis in poly-caprolactone scaffolds with controlled interconnecting pore size.Bioactive materials · 2022Article
- Natural polymer-based scaffolds for soft tissue repair.Frontiers in bioengineering and biotechnology · 2022Review
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- Cartilage repair using stem cells & biomaterials: advancement from bench to bedside.Molecular biology reports · 2020Review
- Pulsed electromagnetic fields potentiate the paracrine function of mesenchymal stem cells for cartilage regeneration.Stem cell research & therapy · 2020Article
- Tuning the biomimetic behavior of scaffolds for regenerative medicine through surface modifications.Journal of tissue engineering and regenerative medicine · 2019Review
- Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields.Scientific reports · 2017Article
- Improvement of In Vitro Three-Dimensional Cartilage Regeneration by a Novel Hydrostatic Pressure Bioreactor.Stem cells translational medicine · 2017Article
- Poly (lactic acid)-based biomaterials for orthopaedic regenerative engineering.Advanced drug delivery reviews · 2016Review
- A controlled double-duration inducible gene expression system for cartilage tissue engineering.Scientific reports · 2016Article
- Simple surface engineering of polydimethylsiloxane with polydopamine for stabilized mesenchymal stem cell adhesion and multipotency.Scientific reports · 2015Article
- Structural properties of scaffolds: Crucial parameters towards stem cells differentiation.World journal of stem cells · 2015Review
- Blends and Nanocomposite Biomaterials for Articular Cartilage Tissue Engineering.Materials (Basel, Switzerland) · 2014Review
- Towards ready-to-use 3-D scaffolds for regenerative medicine: adhesion-based cryopreservation of human mesenchymal stem cells attached and spread within alginate-gelatin cryogel scaffolds.Journal of materials science. Materials in medicine · 2014Article
- Cells behave distinctly within sponges and hydrogels due to differences of internal structure.Tissue engineering. Part A · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Considering the load-bearing physiological requirement of articular cartilage, scaffold for cartilage tissue engineering should exhibit appropriate mechanical responses as natural cartilage undergoing temporary deformation on loading with little structural collapse, and recovering to the original geometry on unloading. A porous elastomeric poly l-lactide-co-ɛ-caprolactone (PLCL) was generated and crosslinked at the surface to chitosan to improve its wettability. Human bone marrow derived mesenchymal stem cells (MSC) attachment, morphological change, proliferation and in vitro cartilage tissue formation on the chitosan-modified PLCL scaffold were compared with the unmodified PLCL scaffold. Chitosan surface promoted more consistent and even distribution of the seeded MSC within the scaffold. MSC rapidly adopted a distinct spread-up morphology on attachment on the chitosan-modified PLCL scaffold with the formation of F-actin stress fiber which proceeded to cell aggregation; an event much delayed in the unmodified PLCL. Enhanced cartilage formation on the chitosan-modified PLCL was shown by real-time PCR analysis, histological and immunochemistry staining and biochemical assays of the cartilage extracellular matrix components. The Young's modulus of the derived cartilage tissues on the chitosan-modified PLCL scaffold was significantly increased and doubled that of the unmodified PLCL. Our results show that chitosan modification of the PLCL scaffold improved the cell compatibility of the PLCL scaffold without significant alteration of the physical elastomeric properties of PLCL and resulted in the formation of cartilage tissue of better quality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.