ArticleFolia microbiologica2011
Comparative study of fungal cell disruption--scope and limitations of the methods.
Article in Folia microbiologica, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 82 citations in OpenAlex.
- Dismantling darkness: interdisciplinary perspectives on melanin degradation.World journal of microbiology & biotechnology · 2026Review
- A novel approach to enhance methane production in anaerobic digestion of waste activated sludge via pre-enrichment by a microbial consortium of degrading fungi.Applied and environmental microbiology · 2026Article
- A Rapid Genomic DNA Extraction Method for Direct Quantitative Polymerase Chain Reaction-Based On-Site Detection of Soil-Borne Fungal Disease.The plant pathology journal · 2026Article
- Engineering Strategies for Fungal Cell Disruption in Biotechnological Applications.Engineering in life sciences · 2025Review
- Beyond dikarya: 28S metabarcoding uncovers cryptic fungal lineages across a tidal estuary.Environmental microbiome · 2025Article
- The ability of selected fungal strains to produce carboxylesterase enzymes for biodegradation and use of bifenthrin insecticide as carbon source: in vitro and in silico approaches.Bioprocess and biosystems engineering · 2024Article
- ClinicalJournal of fungi (Basel, Switzerland) · 2022Review
- Influence of HToxins · 2021Article
- Article
- The Evolving Landscape of Fungal Diagnostics, Current and Emerging Microbiological Approaches.Journal of fungi (Basel, Switzerland) · 2021Review
- Developing Two Rapid Protein Extraction Methods Using Focused-Ultrasonication and Zirconia-Silica Beads for Filamentous Fungi Identification by MALDI-TOF MS.Frontiers in cellular and infection microbiology · 2021Article
- Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA.International journal of molecular sciences · 2020Review
- Optimization of protein isolation by proteomic qualification from Cutaneotrichosporon oleaginosus.Analytical and bioanalytical chemistry · 2020Article
- Investigation of an optimal cell lysis method for the study of the zinc metalloproteome of Histoplasma capsulatum.Analytical and bioanalytical chemistry · 2017Article
- The lung mycobiome in the next-generation sequencing era.Virulence · 2017Review
- Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics.BioMed research international · 2017Review
- Fractionation and identification of the allergic proteins inCurrent medical mycology · 2016Article
- Article
- Production Strategies and Applications of Microbial Single Cell Oils.Frontiers in microbiology · 2016Review
- Evaluation of the efficiency of different disruption methods on yeast cell wall preparation for β-glucan isolation.Molecules (Basel, Switzerland) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simple and effective protocols of cell wall disruption were elaborated for tested fungal strains: Penicillium citrinum, Aspergillus fumigatus, Rhodotorula gracilis. Several techniques of cell wall disintegration were studied, including ultrasound disintegration, homogenization in bead mill, application of chemicals of various types, and osmotic shock. The release of proteins from fungal cells and the activity of a cytosolic enzyme, glucose-6-phosphate dehydrogenase, in the crude extracts were assayed to determine and compare the efficacy of each method. The presented studies allowed adjusting the particular method to a particular strain. The mechanical methods of disintegration appeared to be the most effective for the disintegration of yeast, R. gracilis, and filamentous fungi, A. fumigatus and P. citrinum. Ultrasonication and bead milling led to obtaining fungal cell-free extracts containing high concentrations of soluble proteins and active glucose-6-phosphate dehydrogenase systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.