ArticleDigestive diseases and sciences2012
Soluble mediators released from PI-IBS patients' colon induced alteration of mast cell: involvement of reactive oxygen species.
Article in Digestive diseases and sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Trial
- Proinflammatory Diet Increases the Risk of Irritable Bowel Syndrome: A Prospective Study of 129,408 UK Biobank Participants and Mendelian Randomization Analysis.Digestive diseases and sciences · 2024Article
- Post-infection Irritable Bowel Syndrome.Gastroenterology clinics of North America · 2021Review
- Differences in dietary habits of people withWorld journal of clinical cases · 2021Article
- Establishment and Characterization of a Murine Mucosal Mast Cell Culture Model.International journal of molecular sciences · 2019Article
- Mast Cells in Gut and Brain and Their Potential Role as an Emerging Therapeutic Target for Neural Diseases.Frontiers in cellular neuroscience · 2019Review
- The Protective Effect ofJournal of neurogastroenterology and motility · 2018Article
- Chemical and molecular factors in irritable bowel syndrome: current knowledge, challenges, and unanswered questions.American journal of physiology. Gastrointestinal and liver physiology · 2016Review
- Human Intestinal Barrier Function in Health and Disease.Clinical and translational gastroenterology · 2016Review
- Sevoflurane ameliorates intestinal ischemia-reperfusion-induced lung injury by inhibiting the synergistic action between mast cell activation and oxidative stress.Molecular medicine reports · 2015Article
- Role of gut pathogens in development of irritable bowel syndrome.The Indian journal of medical research · 2014Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGrowing evidence suggests that patients with post-infectious irritable bowel syndrome (PI-IBS) have increased mast cell activation, and that mucosal soluble mediators are involved in the pathophysiology of visceral hyperalgesia. In addition, previous findings show that reactive oxygen species (ROS) and protease-activated receptors (PARs) are mediators of persistent hyperalgesia.
aimsThis article aims to investigate: (1) the ability of soluble factors from colonic biopsies to active peritoneal mast cells (PMCs) in vitro; (2) whether the effects of PMCs degranulation induced by soluble mediators are related to PARs activation; and (3) the ability of phenyl N-tert-butylnitrone (PBN), a ROS scavenger, to modify these alterations.
methodsSupernatant (SUP) from colonic biopsies was collected and applied to PMCs for 12 h. Activation of PMCs was evaluated. The expression of PAR(2) in PMCs was examined by RT-PCR and double-immunofluorescence staining. PBN (10 mM) treatment was administered, then previous alterations were observed again.
resultsStimulation with SUP of PI-IBS led to an increase in activation of PMCs. PAR(2)mRNA expression was significantly increased in PMCs induced by SUP of PI-IBS compared to healthy subjects. After being treated by PBN, the SUP-induced enhancement of PMCs activities could be weakened, and PAR(2)mRNA expression was significantly decreased. A similar result of immunoreactivity for PAR(2) was observed in PMCs.
conclusionsThe study shows that ROS scavenger reverses the SUP of PI-IBS-induced enhancement of PMCs activities, and that these effects may be related to activation of PAR(2). These findings might pave the way to new therapeutic targets in PI-IBS.
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