ArticleClinical and vaccine immunology : CVI2011
Development of an automated and multiplexed serotyping assay for Streptococcus pneumoniae.
Article in Clinical and vaccine immunology : CVI, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Accurate serotype identification ofJournal of clinical microbiology · 2026Article
- Approaches to assess new pneumococcal vaccines for immunogenicity, development and licensure.Human vaccines & immunotherapeutics · 2025Review
- Integrated and high-throughput method to collect, store, recover, and manage microbial isolates in mini-arrays.Microbiology spectrum · 2025Article
- An abundance ofMicrobiology (Reading, England) · 2025Article
- Genome sequences of 36Microbiology resource announcements · 2024Article
- Diagnostic applications of microsphere-based flow cytometry: A review.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- Effect of Oral Streptococci Expressing Pneumococcus-like Cross-Reactive Capsule Types on World Health Organization Recommended Pneumococcal Carriage Detection Procedure.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Phosphorylcholine esterase is critical for Dolichos biflorus and Helix pomatia agglutinin binding to pneumococcal teichoic acid.Journal of basic microbiology · 2020Article
- Pneumococci Can Become Virulent by Acquiring a New Capsule From Oral Streptococci.The Journal of infectious diseases · 2020Article
- Article
- A Common Food Glycan, Pectin, Shares an Antigen with Streptococcus pneumoniae Capsule.mSphere · 2020Article
- Development for Clinical Use of a Multiplexed Immunoassay Using Sputum Samples for Streptococcus pneumoniae: a Non-Culture-Based Approach for Serotype-Specific Detection.Journal of clinical microbiology · 2019Article
- Development of an Extended-Specificity Multiplex Immunoassay for Detection of Streptococcus pneumoniae Serotype-Specific Antigen in Urine by Use of Human Monoclonal Antibodies.Clinical and vaccine immunology : CVI · 2017Article
- The Pneumococcal Serotype 15C Capsule Is Partially O-Acetylated and Allows for Limited Evasion of 23-Valent Pneumococcal Polysaccharide Vaccine-Elicited Anti-Serotype 15B Antibodies.Clinical and vaccine immunology : CVI · 2017Article
- Position of O-Acetylation within the Capsular Repeat Unit Impacts the Biological Properties of Pneumococcal Serotypes 33A and 33F.Infection and immunity · 2017Article
- Discovery of Novel Pneumococcal Serotype 35D, a Natural WciG-Deficient Variant of Serotype 35B.Journal of clinical microbiology · 2017Article
- Comparison of sequential multiplex PCR, sequetyping and whole genome sequencing for serotyping of Streptococcus pneumoniae.PloS one · 2017Article
- Monoclonal Antibodies to Shigella Lipopolysaccharide Are Useful for Vaccine Production.Clinical and vaccine immunology : CVI · 2016Article
- Development of a TaqMan Array Card for Pneumococcal Serotyping on Isolates and Nasopharyngeal Samples.Journal of clinical microbiology · 2016Article
- Polyamine transporter in Streptococcus pneumoniae is essential for evading early innate immune responses in pneumococcal pneumonia.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Streptococcus pneumoniae expresses more than 90 capsule types, and currently available pneumococcal vaccines are designed to provide serotype-specific protection. Consequently, serotyping of pneumococcal isolates is important for determining the serotypes to be included in pneumococcal vaccines and to monitor their efficacy. Yet serotyping of pneumococcal isolates has remained a significant technical challenge. By multiplexing many assays, we have now developed a simple yet comprehensive serotyping assay system that can not only identify all known pneumococcal serotypes but also subdivide nontypeable (NT) isolates into those with or without the conventional capsule locus. We have developed this assay system to require only six key reagents: two are used in one multiplex inhibition-type immunoassay, and four are required in two multiplex PCR-based assays. The assay system is largely automated by a seamless combination of monoclonal antibody-based and PCR-based multiplex assays using the flow cytometric bead array technology from Luminex. The assay system has been validated with a panel of pneumococci expressing all known pneumococcal serotypes and was found to be easily transferable to another laboratory.
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Registered trials
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