ArticleJournal of neurophysiology2011
Long-duration perforated patch recordings from spinal interneurons of adult mice.
Article in Journal of neurophysiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Machine-learning classification of motor unit types in the adult mouse.The Journal of physiology · 2026Article
- Article
- Machine-Learning Classification of Motor Unit Types in the Adult Mouse.bioRxiv : the preprint server for biology · 2025Article
- ElecFeX is a user-friendly toolbox for efficient feature extraction from single-cell electrophysiological recordings.Cell reports methods · 2024Article
- Effect of size on expression of bistability in mouse spinal motoneurons.Journal of neurophysiology · 2024Article
- Modulation of SK Channels via Calcium Buffering Tunes Intrinsic Excitability of Parvalbumin Interneurons in Neuropathic Pain: A Computational and Experimental Investigation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Neural Stem Cell Grafts Form Extensive Synaptic Networks that Integrate with Host Circuits after Spinal Cord Injury.Cell stem cell · 2020Article
- Role of Human-Induced Pluripotent Stem Cell-Derived Spinal Cord Astrocytes in the Functional Maturation of Motor Neurons in a Multielectrode Array System.Stem cells translational medicine · 2019Article
- Molecular and electrophysiological properties of mouse motoneuron and motor unit subtypes.Current opinion in physiology · 2019Article
- Segregation of glutamatergic and cholinergic transmission at the mixed motoneuron Renshaw cell synapse.Scientific reports · 2017Article
- Decerebrate mouse model for studies of the spinal cord circuits.Nature protocols · 2017Article
- Adult spinal V2a interneurons show increased excitability and serotonin-dependent bistability.Journal of neurophysiology · 2015Article
- Functional subpopulations of V3 interneurons in the mature mouse spinal cord.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Simultaneous intracellular recording of a lumbar motoneuron and the force produced by its motor unit in the adult mouse in vivo.Journal of visualized experiments : JoVE · 2012Article
- Neuronal activity in the isolated mouse spinal cord during spontaneous deletions in fictive locomotion: insights into locomotor central pattern generator organization.The Journal of physiology · 2012Article
- Postnatal emergence of serotonin-induced plateau potentials in commissural interneurons of the mouse spinal cord.Journal of neurophysiology · 2012Article
- Spinal cord injury induces serotonin supersensitivity without increasing intrinsic excitability of mouse V2a interneurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- A gradient in endogenous rhythmicity and oscillatory drive matches recruitment order in an axial motor pool.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- A comparison of serotonin neuromodulation of mouse spinal V2a interneurons using perforated patch and whole cell recording techniques.Frontiers in cellular neuroscience · 2012Article
- Long-term patch recordings from adult spinal neurons herald new era of opportunity.Journal of neurophysiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
It has been very difficult to record from interneurons in acute slices of the lumbar spinal cord from mice >3 wk of age. The low success rate and short recording times limit in vitro experimentation on mouse spinal networks to neonatal and early postnatal periods when locomotor networks are still developmentally immature. To overcome this limitation and enable investigation of mature locomotor network neurons, we have established a reliable procedure to record from spinal cord neurons in slices from adult, behaviorally mature mice of any age. Two key changes to the established neonate procedure were implemented. First, we remove the cord by a dorsal laminectomy from a deeply anesthetized animal. This enables respiration and other vital functions to continue up to the moment the maximally oxygenated lumbar spinal cord is removed, improving the health of the slices. Second, since adult spinal cord interneurons appear more sensitive to the intracellular dialysis that occurs during whole cell recordings, we introduced perforated patch recordings to the procedure. Stable recordings up to 12 h in duration were obtained with our new method. This will allow investigation of changes in mature neuronal properties in disease states or after spinal cord injury and allow prolonged recordings of responses to drug application that were previously impossible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.