ArticleMolecular biology of the cell2011
Coordinated regulation of sulfur and phospholipid metabolism reflects the importance of methylation in the growth of yeast.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
- Evolution of the Cell-Cycle Clock Models: Biochemical or Transcriptional?Advances in experimental medicine and biology · 2026Review
- Breeding of Saccharomyces cerevisiae with a High-Throughput Screening Strategy for Improvement of S-Adenosyl-L-Methionine Production.Applied biochemistry and biotechnology · 2024Article
- Yeast Rim11 kinase responds to glutathione-induced stress by regulating the transcription of phospholipid biosynthetic genes.Molecular biology of the cell · 2024Article
- The Swi-Snf chromatin remodeling complex mediates gene repression through metabolic control.Nucleic acids research · 2023Article
- Folate-mediated one-carbon metabolism as a potential antifungal target for the sustainable cultivation of microalga Haematococcus pluvialis.Biotechnology for biofuels and bioproducts · 2023Article
- A Toolkit for Precise, Multigene Control inACS synthetic biology · 2022Article
- Review
- Gene by Environment Interactions reveal new regulatory aspects of signaling network plasticity.PLoS genetics · 2022Article
- Shortening of membrane lipid acyl chains compensates for phosphatidylcholine deficiency in choline-auxotroph yeast.The EMBO journal · 2021Article
- Distinct pattern of one-carbon metabolism, a nutrient-sensitive pathway, in invasive breast cancer: A metabolomic study.Oncotarget · 2020Article
- Network dynamics of the yeast methyltransferome.Microbial cell (Graz, Austria) · 2019Article
- Phospholipids and inositol phosphates linked to the epigenome.Histochemistry and cell biology · 2018Review
- Common and divergent features of galactose-1-phosphate and fructose-1-phosphate toxicity in yeast.Molecular biology of the cell · 2018Article
- A Metabolic Function for Phospholipid and Histone Methylation.Molecular cell · 2017Article
- Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains.Microbial cell factories · 2016Article
- Correlation between Low Temperature Adaptation and Oxidative Stress in Saccharomyces cerevisiae.Frontiers in microbiology · 2016Article
- Article
- Changes in SAM2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae.Microbial cell factories · 2014Article
- Multiple inputs control sulfur-containing amino acid synthesis in Saccharomyces cerevisiae.Molecular biology of the cell · 2014Article
- The response to inositol: regulation of glycerolipid metabolism and stress response signaling in yeast.Chemistry and physics of lipids · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
A yeast strain lacking Met4p, the primary transcriptional regulator of the sulfur assimilation pathway, cannot synthesize methionine. This apparently simple auxotroph did not grow well in rich media containing excess methionine, forming small colonies on yeast extract/peptone/dextrose plates. Faster-growing large colonies were abundant when overnight cultures were plated, suggesting that spontaneous suppressors of the growth defect arise with high frequency. To identify the suppressor mutations, we used genome-wide single-nucleotide polymorphism and standard genetic analyses. The most common suppressors were loss-of-function mutations in OPI1, encoding a transcriptional repressor of phospholipid metabolism. Using a new system that allows rapid and specific degradation of Met4p, we could study the dynamic expression of all genes following loss of Met4p. Experiments using this system with and without Opi1p showed that Met4 activates and Opi1p represses genes that maintain levels of S-adenosylmethionine (SAM), the substrate for most methyltransferase reactions. Cells lacking Met4p grow normally when either SAM is added to the media or one of the SAM synthetase genes is overexpressed. SAM is used as a methyl donor in three Opi1p-regulated reactions to create the abundant membrane phospholipid, phosphatidylcholine. Our results show that rapidly growing cells require significant methylation, likely for the biosynthesis of phospholipids.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.