ArticleMolecular biology of the cell2011
The filament-forming protein Pil1 assembles linear eisosomes in fission yeast.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 78 citations in OpenAlex.
- Fission yeast Pdk1 kinase regulates cytokinesis and eisosomes.Molecular biology of the cell · 2025Article
- Fission yeast Pdk1 kinase regulates cytokinesis and eisosomes.bioRxiv : the preprint server for biology · 2025Article
- The Predicted Structure ofJournal of fungi (Basel, Switzerland) · 2025Article
- Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in the Fission Yeast Schizosaccharomyces pombe.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Elevated levels of sphingolipid MIPC in the plasma membrane disrupt the coordination of cell growth with cell wall formation in fission yeast.PLoS genetics · 2023Article
- VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells.Plant methods · 2023Article
- The cytoplasmic tail of the mechanosensitive channel Pkd2 regulates its internalization and clustering in eisosomes.Journal of cell science · 2023Article
- Microdomain Protein Nce102 Is a Local Sensor of Plasma Membrane Sphingolipid Balance.Microbiology spectrum · 2022Article
- MaSln1, a Conserved Histidine Protein Kinase, Contributes to Conidiation Pattern Shift Independent of the MAPK Pathway inMicrobiology spectrum · 2022Article
- Plasma Membrane MCC/Eisosome Domains Promote Stress Resistance in Fungi.Microbiology and molecular biology reviews : MMBR · 2020Review
- Plasma Membrane Protein Nce102 Modulates Morphology and Function of the Yeast Vacuole.Biomolecules · 2020Article
- Atg1 kinase in fission yeast is activated by Atg11-mediated dimerization and cis-autophosphorylation.eLife · 2020Article
- Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition.The Journal of cell biology · 2020Article
- The Composition and the Structure of MCC/Eisosomes inFrontiers in microbiology · 2020Article
- Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis.eLife · 2019Article
- Human myosin 1e tail but not motor domain replaces fission yeast Myo1 domains to support myosin-I function during endocytosis.Experimental cell research · 2019Article
- The TOR pathway modulates cytoophidium formation inThe Journal of biological chemistry · 2019Article
- Role of MCC/Eisosome in Fungal Lipid Homeostasis.Biomolecules · 2019Review
- Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion.Molecular biology of the cell · 2018Article
- Eisosomes.Current biology : CB · 2018Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The cortical cytoskeleton mediates a range of cellular activities such as endocytosis, cell motility, and the maintenance of cell rigidity. Traditional polymers, including actin, microtubules, and septins, contribute to the cortical cytoskeleton, but additional filament systems may also exist. In yeast cells, cortical structures called eisosomes generate specialized domains termed MCCs to cluster specific proteins at sites of membrane invaginations. Here we show that the core eisosome protein Pil1 forms linear cortical filaments in fission yeast cells and that purified Pil1 assembles into filaments in vitro. In cells, Pil1 cortical filaments are excluded from regions of cell growth and are independent of the actin and microtubule cytoskeletons. Pil1 filaments assemble slowly at the cell cortex and appear stable by time-lapse microscopy and fluorescence recovery after photobleaching. This stability does not require the cell wall, but Pil1 and the transmembrane protein Fhn1 colocalize and are interdependent for localization to cortical filaments. Increased Pil1 expression leads to cytoplasmic Pil1 rods that are stable and span the length of cylindrical fission yeast cells. We propose that Pil1 is a novel component of the yeast cytoskeleton, with implications for the role of filament assembly in the spatial organization of cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.