ArticleThe Journal of biological chemistry2011
Paxillin enables attachment-independent tyrosine phosphorylation of focal adhesion kinase and transformation by RAS.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 15 citations in OpenAlex.
- The role of RAS oncogenes in controlling epithelial mechanics.Trends in cell biology · 2023Review
- Comprehensive understanding of anchorage-independent survival and its implication in cancer metastasis.Cell death & disease · 2021Review
- Association of papillomavirus E6 proteins with either MAML1 or E6AP clusters E6 proteins by structure, function, and evolutionary relatedness.PLoS pathogens · 2017Article
- Cool-associated Tyrosine-phosphorylated Protein 1 Is Required for the Anchorage-independent Growth of Cervical Carcinoma Cells by Binding Paxillin and Promoting AKT Activation.The Journal of biological chemistry · 2017Article
- Paxillin: a crossroad in pathological cell migration.Journal of hematology & oncology · 2017Review
- Cbl-b promotes cell detachment via ubiquitination of focal adhesion kinase.Oncology letters · 2016Article
- Roles of paxillin family members in adhesion and ECM degradation coupling at invadosomes.The Journal of cell biology · 2016Article
- GIT1 promotes lung cancer cell metastasis through modulating Rac1/Cdc42 activity and is associated with poor prognosis.Oncotarget · 2015Article
- MEKK2 regulates paxillin ubiquitylation and localization in MDA-MB 231 breast cancer cells.The Biochemical journal · 2014Article
- Interactions between E6, FAK, and GIT1 at paxillin LD4 are necessary for transformation by bovine papillomavirus 1 E6.Journal of virology · 2014Article
- The role of FAK in tumor metabolism and therapy.Pharmacology & therapeutics · 2014Review
- mTORC2 regulates mechanically induced cytoskeletal reorganization and lineage selection in marrow-derived mesenchymal stem cells.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- Annexin A1, formyl peptide receptor, and NOX1 orchestrate epithelial repair.The Journal of clinical investigation · 2013Article
- The adaptor protein and Arf GTPase-activating protein Cat-1/Git-1 is required for cellular transformation.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Paxillin and HIC5 are closely related adapter proteins that regulate cell migration and are tyrosine-phosphorylated by focal adhesion kinase (FAK). Paxillin, HIC5, and FAK tyrosine phosphorylation increase upon cell attachment and decrease upon detachment from extracellular matrix. Unexpectedly, we found that although FAK tyrosine phosphorylation in attached cells did not require paxillin, in detached fibroblasts there was remaining FAK tyrosine phosphorylation that required expression of paxillin and was not supported by HIC5. The support of attachment-independent FAK tyrosine phosphorylation required the paxillin LIM domains and suggested that paxillin might facilitate oncogenic transformation. Paxillin but not HIC5 augmented anchorage-independent cell proliferation induced by RAS. Both anchorage-independent FAK tyrosine phosphorylation and RAS-induced colony formation required multiple docking sites on paxillin, including LD4 (docking sites for FAK-Src and GIT1/2-PIX-NCK-PAK complex), LD5, and all four carboxyl-terminal LIM domains (that bind tubulin and PTP-PEST). Analysis using paxillin mutants dissociated domains of paxillin that are required for regulation of cell migration from domains that are required for anchorage-independent cell proliferation and demonstrated essential functions of the paxillin LIM domains that are not found in HIC5 LIM domains. These results highlight the role of paxillin in facilitating attachment-independent signal transduction implicated in cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.