ArticleBMC research notes2011
PCR could be a method of choice for identification of both pulmonary and extra-pulmonary tuberculosis.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
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- Diagnosis and Treatment of Skipped Multifocal Spinal Tuberculosis Lesions.Orthopaedic surgery · 2023Review
- Comparative Performance of Line Probe Assay and GeneXpert in the Detection of Rifampicin Monoresistance in a TB-Endemic African Country.Antibiotics (Basel, Switzerland) · 2022Article
- Analysis of Tuberculosis Meningitis Pathogenesis, Diagnosis, and Treatment.Journal of clinical medicine · 2020Review
- Pulmonary tuberculosis presenting as lung abscess in a 10-month-Old infant.Respiratory medicine case reports · 2020Article
- Diagnosing tuberculosis with a novel support vector machine-based artificial immune recognition system.Iranian Red Crescent medical journal · 2015Article
- A polymerase chain reaction and enzyme linked immunosorbent assay based approach for diagnosis and differentiation between vaccinated and infected cattle with Mycobacterium bovis.Journal of pharmacy & bioallied sciences · 2014Article
- Polymerase chain reaction targeting insertion sequence for the diagnosis of extrapulmonary tuberculosis.The Indian journal of medical research · 2014Article
- Evaluation of six different DNA extraction methods for detection of Mycobacterium tuberculosis by means of PCR-IS6110: preliminary study.BMC research notes · 2013Article
- PCR as a diagnostic tool for extra-pulmonary tuberculosis.Journal of clinical and diagnostic research : JCDR · 2013Article
- Tuberculous spondylitis presenting as severe chest pain.Clinics and practice · 2012Article
- Microbiological diagnosis of spinal tuberculosis.International orthopaedics · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNucleic acid amplification assays including PCR have revolutionized the detection of Mycobacterium tuberculosis (MTB). Tuberculosis spread to almost every organ of the body and is characterized on the basis of localization of infection. Therefore, different types of body fluids and tissues can be used for the detection of MTB.From 2004 to 2010 total 766 different types of smear negative samples from patients, clinically suspected for tuberculosis were received and investigated at Division of Molecular Diagnostics, University of the Punjab Lahore for the diagnosis of tuberculosis. Mycobacterial DNA was extracted followed by PCR amplification.
findingsA total of 356 (46.5%) samples were found positive by PCR for MTB. These included; serum (4.8%), blood (36.3%), urine (46.6%), cerebro spinal fluid (CSF) (42.1%), ascetic fluid (67.6%), pleural fluid (52%), pericardial fluid (30%), pus (38.6%), bone marrow (60%), sputum (38.8%) and bronchoalveolar lavage (BAL) (70%). Over all there was no significant difference in males and females neither in different age groups for the identification of MTB.
conclusionWe conclude that PCR is a useful and sensitive tool for the early diagnosis of MTB in variety of clinical samples.
Identifiers
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Registered trials
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