Evidence map›Paper›PMID 21899740›Full record

ArticleVirology journal2011

Characterization of a ViI-like phage specific to Escherichia coli O157:H7.

Elizabeth M Kutter, Kyobi Skutt-Kakaria, Bob Blasdel, Ayman El-Shibiny, Anna Castano, Daniel Bryan, Andrew M Kropinski, Andre Villegas, Hans-Wolfgang Ackermann, Ana L Toribio and 4 more

Open access · goldAbstract read
In one paragraph

Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
6.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 70 citations in OpenAlex.

  1. Article
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  6. Article
  7. Engineering ofCells · 2023
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  13. Review
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  15. Subtypes of tail spike proteins predicts the host range ofComputational and structural biotechnology journal · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 8 institutions in 4 countries.

Elizabeth M KutterThe Evergreen State College, Olympia, WA, USA. kutterb@evergreen.edu
Kyobi Skutt-Kakaria
Bob Blasdel
Ayman El-Shibiny
Anna Castano
Daniel Bryan
Andrew M Kropinski
Andre Villegas
Hans-Wolfgang Ackermann
Ana L Toribio
Derek Pickard
Hany Anany
Todd Callaway
Andrew D Brabban
The Evergreen State College · USPublic Health Agency of Canada · CAWellcome Sanger Institute · GBAin Shams University · EGThe Ohio State University · USUnited States Department of Agriculture · USUniversité Laval · CAUniversity of Colorado Denver · US

Funding

T-Even Phages for Food-Chain Biocontrol of e.coli 0157R15GM063637 · NIGMS · EVERGREEN STATE COLLEGE · PI BRABBAN, ANDREW DAVID, KUTTER, ELIZABETH MARTIN · 2001 to 2009
$526k
NIGMS NIH HHS 2-R15GM063637-02NIGMS NIH HHS 2R15GM63637-3A1.
6 · The paper itself

Abstract

Phage vB_EcoM_CBA120 (CBA120), isolated against Escherichia coli O157:H7 from a cattle feedlot, is morphologically very similar to the classic phage ViI of Salmonella enterica serovar Typhi. Until recently, little was known genetically or physiologically about the ViI-like phages, and none targeting E. coli have been described in the literature. The genome of CBA120 has been fully sequenced and is highly similar to those of both ViI and the Shigella phage AG3. The core set of structural and replication-related proteins of CBA120 are homologous to those from T-even phages, but generally are more closely related to those from T4-like phages of Vibrio, Aeromonas and cyanobacteria than those of the Enterobacteriaceae. The baseplate and method of adhesion to the host are, however, very different from those of either T4 or the cyanophages. None of the outer baseplate proteins are conserved. Instead of T4's long and short tail fibers, CBA120, like ViI, encodes tail spikes related to those normally seen on podoviruses. The 158 kb genome, like that of T4, is circularly permuted and terminally redundant, but unlike T4 CBA120 does not substitute hmdCyt for cytosine in its DNA. However, in contrast to other coliphages, CBA120 and related coliphages we have isolated cannot incorporate 3H-thymidine (3H-dThd) into their DNA. Protein sequence comparisons cluster the putative "thymidylate synthase" of CBA120, ViI and AG3 much more closely with those of Delftia phage φW-14, Bacillus subtilis phage SPO1, and Pseudomonas phage YuA, all known to produce and incorporate hydroxymethyluracil (hmdUra).

Indexed as

ColiphagesGenome, ViralAnimalsBiological EvolutionCattleComputational BiologyDNA FingerprintingEscherichia coli O157Microscopy, Electron, TransmissionPhylogenyPolysaccharides, BacterialSequence Analysis, DNAThymidineTritiumViral ProteinsVirus ReplicationPolysaccharides, BacterialThymidineTritiumViral Proteins

Identifiers

PMID21899740
PMCPMC3184105
OpenAlexW2107878343

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.