ArticleStem cells (Dayton, Ohio)2011
Site-specific recombinase strategy to create induced pluripotent stem cells efficiently with plasmid DNA.
Article in Stem cells (Dayton, Ohio), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Programmable enzymes for targeted gene insertion.Nature reviews. Genetics · 2026Review
- Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.Nature biotechnology · 2023Article
- Microneedle systems for delivering nucleic acid drugs.Journal of pharmaceutical investigation · 2022Review
- Application of iPSC to Modelling of Respiratory Diseases.Advances in experimental medicine and biology · 2020Review
- Nucleic acid delivery to mesenchymal stem cells: a review of nonviral methods and applications.Journal of biological engineering · 2019Review
- Current Therapeutic Strategies for Stem Cell-Based Cartilage Regeneration.Stem cells international · 2018Review
- Blastocyst Formation Rate and Transgene Expression are Associated with Gene Insertion into Safe and Non-Safe Harbors in the Cattle Genome.Scientific reports · 2017Article
- Pluripotent stem cells to hepatocytes, the journey so far.Biomedical reports · 2017Article
- Current Advances and Limitations in Modeling ALS/FTD in a Dish Using Induced Pluripotent Stem Cells.Frontiers in neuroscience · 2017Review
- A Compendium of Preparation and Application of Stem Cells in Parkinson's Disease: Current Status and Future Prospects.Frontiers in aging neuroscience · 2016Review
- Production of transgenic cattle highly expressing human serum albumin in milk by phiC31 integrase-mediated gene delivery.Transgenic research · 2015Article
- Simple derivation of transgene-free iPS cells by a dual recombinase approach.Molecular biotechnology · 2014Article
- Stem cell imaging: from bench to bedside.Cell stem cell · 2014Review
- Recombinase-mediated reprogramming and dystrophin gene addition in mdx mouse induced pluripotent stem cells.PloS one · 2014Article
- Zinc finger nuclease-expressing baculoviral vectors mediate targeted genome integration of reprogramming factor genes to facilitate the generation of human induced pluripotent stem cells.Stem cells translational medicine · 2013Article
- Generation and genetic engineering of human induced pluripotent stem cells using designed zinc finger nucleases.Stem cells and development · 2013Article
- Non-invasive in-vivo imaging of stem cells after transplantation in cardiovascular tissue.Theranostics · 2013Review
- A site-specific recombinase-based method to produce antibiotic selectable marker free transgenic cattle.PloS one · 2013Article
- Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector.PloS one · 2013Article
- Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Induced pluripotent stem cells (iPSCs) have revolutionized the stem cell field. iPSCs are most often produced by using retroviruses. However, the resulting cells may be ill-suited for clinical applications. Many alternative strategies to make iPSCs have been developed, but the nonintegrating strategies tend to be inefficient, while the integrating strategies involve random integration. Here, we report a facile strategy to create murine iPSCs that uses plasmid DNA and single transfection with sequence-specific recombinases. PhiC31 integrase was used to insert the reprogramming cassette into the genome, producing iPSCs. Cre recombinase was then used for excision of the reprogramming genes. The iPSCs were demonstrated to be pluripotent by in vitro and in vivo criteria, both before and after excision of the reprogramming cassette. This strategy is comparable with retroviral approaches in efficiency, but is nonhazardous for the user, simple to perform, and results in nonrandom integration of a reprogramming cassette that can be readily deleted. We demonstrated the efficiency of this reprogramming and excision strategy in two accessible cell types, fibroblasts and adipose stem cells. This simple strategy produces pluripotent stem cells that have the potential to be used in a clinical setting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.