ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2011
1,25-dihydroxyvitamin D3 influences cellular homocysteine levels in murine preosteoblastic MC3T3-E1 cells by direct regulation of cystathionine β-synthase.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 112 citations in OpenAlex.
- Using metabolic profiling and gene expression analyses to explore molecular effects of replacing saturated fat with polyunsaturated fat-a randomized controlled dietary intervention study.The American journal of clinical nutrition · 2019Trial
- The association of 25(OH)D with blood pressure, pulse pressure and carotid-radial pulse wave velocity in African women.PloS one · 2013Trial
- Nonlinear associations between serum vitamin mixtures and cardiovascular disease risk: insights from a national cross-sectional analysis.European journal of medical research · 2026Article
- Relationship between plasma homocysteine and clinical grading of varicocele.Asian journal of andrology · 2025Article
- Steroids and One-Carbon Metabolism: Clinical Implications in Endocrine Disorders.Neuroendocrinology · 2025Review
- Association between MTHFR polymorphisms and vitamin D status in infertile women: a mediation analysis.Frontiers in nutrition · 2025Article
- Association between Homocysteine and Vitamin D Levels in Asymptomatic Korean Adults.Nutrients · 2024Article
- Impact of Folate Intake on Bone Mineral Density in Patients with Inflammatory Bowel Disease.Nutrients · 2023Article
- The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice.Scientific reports · 2023Article
- Serum 25-hydroxyvitamin D level is associated with short-term glycemic variability metrics derived from continuous glucose monitoring in T2DM.Scientific reports · 2023Article
- Associations of serum 25-hydroxyvitamin D with serum folate, cobalanin, and homocysteine concentrations and methylene tetrahydrofolate reductase (MTHFR) gene polymorphisms in healthy adults.Hormones (Athens, Greece) · 2023Article
- Article
- The evolution of folate supplementation - from one size for all to personalized, precision, poly-paths.Journal of translational internal medicine · 2023Article
- Genomic mechanisms controlling renal vitamin D metabolism.The Journal of steroid biochemistry and molecular biology · 2023Article
- A Metabolic Paradigm for Hydrogen Sulfide SignalingAntioxidants & redox signaling · 2023Review
- Consumption of very low-mineral water may threaten cardiovascular health by increasing homocysteine in children.Frontiers in nutrition · 2023Article
- Serum 25-hydroxyvitamin DNutrition research and practice · 2022Article
- Modulation of cardiometabolic risk and CardioRenal syndrome by oral vitamin DRenal failure · 2022Article
- Serum Homocysteine and Vascular Calcification: Advances in Mechanisms, Related Diseases, and Nutrition.Korean journal of family medicine · 2022Article
- Association of serum 25-hydroxyvitamin D (25(OH)D) levels with the gut microbiota and metabolites in postmenopausal women in China.Microbial cell factories · 2022Article
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Authors and funding
14 authors at 5 institutions in 4 countries.
Funding
Abstract
High homocysteine (HCY) levels are a risk factor for osteoporotic fracture. Furthermore, bone quality and strength are compromised by elevated HCY owing to its negative impact on collagen maturation. HCY is cleared by cystathionine β-synthase (CBS), the first enzyme in the transsulfuration pathway. CBS converts HCY to cystathionine, thereby committing it to cysteine synthesis. A microarray experiment on MC3T3-E1 murine preosteoblasts treated with 1,25-dihydroxyvitamin D(3) [1,25(OH)(2) D(3) ] revealed a cluster of genes including the cbs gene, of which the transcription was rapidly and strongly induced by 1,25(OH)(2) D(3) . Quantitative real-time PCR and Western blot analysis confirmed higher levels of cbs mRNA and protein after 1,25(OH)(2) D(3) treatment in murine and human cells. Moreover, measurement of CBS enzyme activity and quantitative measurements of HCY, cystathionine, and cysteine concentrations were consistent with elevated transsulfuration activity in 1,25(OH)(2) D(3) -treated cells. The importance of a functional vitamin D receptor (VDR) for transcriptional regulation of cbs was shown in primary murine VDR knockout osteoblasts, in which upregulation of cbs in response to 1,25(OH)(2) D(3) was abolished. Chromatin immunoprecipitation on chip and transfection studies revealed a functional vitamin D response element in the second intron of cbs. To further explore the potential clinical relevance of our ex vivo findings, human data from the Longitudinal Aging Study Amsterdam suggested a correlation between vitamin D status [25(OH)D(3) levels] and HCY levels. In conclusion, this study showed that cbs is a primary 1,25(OH)(2) D(3) target gene which renders HCY metabolism responsive to 1,25(OH)(2) D(3).
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