ArticleCell adhesion & migration
Overexpression of VEGF189 in breast cancer cells induces apoptosis via NRP1 under stress conditions.
Article in Cell adhesion & migration. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Impact of ligand binding on VEGFR1, VEGFR2, and NRP1 localization in human endothelial cells.PLoS computational biology · 2025Article
- Obesity Alters the Vascular Morphology and VEGF-A Signaling in Adipose Tissue.FASEB bioAdvances · 2025Article
- Neuropilin-1: A Multifaceted Target for Cancer Therapy.Current oncology (Toronto, Ont.) · 2025Review
- Evaluation of tumorigenesis-related miRNAs in breast cancer in Egyptian women: a retrospective, exploratory analysis.Scientific reports · 2024Article
- How VEGF-A and its splice variants affect breast cancer development - clinical implications.Cellular oncology (Dordrecht, Netherlands) · 2022Review
- N6-methylandenosine-related immune genes correlate with prognosis and immune landscapes in gastric cancer.Frontiers in oncology · 2022Article
- VEGF-A121a binding to Neuropilins - A concept revisited.Cell adhesion & migration · 2018Review
- Article
- A computational analysis of in vivo VEGFR activation by multiple co-expressed ligands.PLoS computational biology · 2017Article
- Antiangiogenic Therapy for Diabetic Nephropathy.BioMed research international · 2017Review
- NRP1 function and targeting in neurovascular development and eye disease.Progress in retinal and eye research · 2016Review
- MiR-148a functions to suppress metastasis and serves as a prognostic indicator in triple-negative breast cancer.Oncotarget · 2016Article
- MDA-MB-231 breast cancer cells overexpressing single VEGF isoforms display distinct colonisation characteristics.British journal of cancer · 2015Article
- Control of cellular motility by neuropilin-mediated physical interactions.Biomolecular concepts · 2014Review
- Extracellular regulation of VEGF: isoforms, proteolysis, and vascular patterning.Cytokine & growth factor reviews · 2014Review
- Tumour cells expressing single VEGF isoforms display distinct growth, survival and migration characteristics.PloS one · 2014Article
- Autocrine functions of VEGF in breast tumor cells: adhesion, survival, migration and invasion.Cell adhesion & migrationReview
- Enhancing integrin function by VEGF/neuropilin signaling: implications for tumor biology.Cell adhesion & migrationReview
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The existence of multiple VEGF-A isoforms raised the possibility that they may have distinct functions in tumor growth. We have previously published that VEGF189 and VEGF165 contribute to breast cancer progression and angiogenesis, but VEGF165 induced the most rapid tumor uptake. Since VEGF165 has been described as a survival factor for breast tumor cells, we questioned here the effects of VEGF189 on the survival/apoptosis of MDA-MB-231 cells. We used clones which overexpress VEGF189 (V189) or VEGF165 (V165) isoforms and compared them to a control one (cV). Overexpression of VEGF189 resulted in increased cell apoptosis, as determined by Annexin-V apoptosis assay, under serum starvation and doxorubicin treatment, while VEGF 165 was confirmed to be a survival factor. Since MDA-MB-231 highly express NRP1 (a co-receptor for VEGF-A), we used short hairpin RNA (shRNA) to knockdown NRP1 expression. V189shNRP1 clones were characterized by reduced apoptosis and higher necrosis, as compared to V189shCtl, under stress conditions. Unexpectedly, NRP1 knock-down had no effect on the survival or apoptosis of V165 cells. VEGF189 showed greater affinity towards NRP1 than VEGF165 using a BIAcore binding assay. Finally, since endogenously produced urokinase-type plasminogen (uPA) has been found to prevent apoptosis in breast cancers, we analyzed the level of uPA activity in our clones. An inhibition of uPA activity was observed in V189shNRP1 clones. Altogether, these results suggest a major role of NRP1 in apoptosis induced by VEGF189 in stress conditions and confirm VEGF165 as a survival factor.
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