ArticleMolecular and cellular biology2011
Essential roles of ECAT15-2/Dppa2 in functional lung development.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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28 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Pluripotent stem cells: induction and self-renewal.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Pooled it
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- Embryonic stem cell factors DPPA2/4 amplify active H3K4me3-H2AK119ub chromatin domains in non-small cell lung cancer.Genes & development · 2026Article
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- Epigenetic Reprogramming in Early Animal Development.Cold Spring Harbor perspectives in biology · 2022Review
- Capturing Pluripotency and Beyond.Cells · 2021Review
- DPPA2 and DPPA4 are dispensable for mouse zygotic genome activation and pre-implantation development.Development (Cambridge, England) · 2021Article
- Maternal Dppa2 and Dppa4 are dispensable for zygotic genome activation but important for offspring survival.Development (Cambridge, England) · 2021Article
- DPPA2, DPPA4, and other DPPA factor epigenomic functions in cell fate and cancer.Stem cell reports · 2021Review
- Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genes.Cell reports · 2021Article
- Epigenetic priming by Dppa2 and 4 in pluripotency facilitates multi-lineage commitment.Nature structural & molecular biology · 2020Article
- Dppa2 and Dppa4 safeguard bivalent chromatin in order to establish a pluripotent epigenome.Nature structural & molecular biology · 2020Article
- Dppa2 and Dppa4 counteract de novo methylation to establish a permissive epigenome for development.Nature structural & molecular biology · 2020Article
- DUX is a non-essential synchronizer of zygotic genome activation.Development (Cambridge, England) · 2020Article
- Get Out and Stay Out: New Insights Into DNA Methylation Reprogramming in Mammals.Frontiers in cell and developmental biology · 2020Review
- The necessity of ZSCAN4 for preimplantation development and gene expression of bovine embryos.The Journal of reproduction and development · 2019Article
- DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program.PLoS biology · 2019Article
- DPPA2 and DPPA4 are necessary to establish a 2C-like state in mouse embryonic stem cells.EMBO reports · 2019Article
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- Dppa2 and Dppa4 directly regulate the Dux-driven zygotic transcriptional program.Genes & development · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many transcription factors and DNA binding proteins play essential roles in the development of organs in which they are highly and/or specifically expressed. Embryonic stem cell (ESC)-associated transcript 15-1 (ECAT15-1) and ECAT15-2, also known as developmental pluripotency-associated 4 (Dppa4) and Dppa2, respectively, are enriched in mouse ESCs and preimplantation embryos, and their genes encode homologous proteins with a common DNA binding domain known as the SAP motif. Previously, ECAT15-1 was shown to be important in lung development, while it is dispensable in early development. In this study, we generated ECAT15-2 single and ECAT15-1 ECAT15-2 double knockout (double KO) mice and found that almost all mutants, like ECAT15-1 mutants, died around birth with respiratory defects. Paradoxically, the expression of neither ECAT15-1 nor ECAT15-2 was detected in lung organogenesis. Several genes, such as Nkx2-5, Gata4, and Pitx2, were downregulated in the ECAT15-2-null lung. On the other hand, genomic DNA of these genes showed inactive chromatin statuses in ECAT15-2-null ESCs, but not in wild-type ESCs. The chromatin immunoprecipitation (ChIP) assay revealed that ECAT15-2 binds to the regulatory region of Nkx2-5 in ESCs. These data suggest that ECAT15-2 has important roles in lung development, where it is no longer expressed, by leaving epigenetic marks from earlier developmental stages.
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