ArticleRespiratory research2011
Mucin granule-associated proteins in human bronchial epithelial cells: the airway goblet cell "granulome".
Article in Respiratory research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Weighted gene co-expression network analysis and whole genome sequencing identify potential lung cancer biomarkers.Frontiers in oncology · 2024Article
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- MARCKS and Lung Disease.American journal of respiratory cell and molecular biology · 2019Review
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- CLCA1 and TMEM16A: the link towards a potential cure for airway diseases.Expert review of respiratory medicine · 2015Review
- Novel Roles for Chloride Channels, Exchangers, and Regulators in Chronic Inflammatory Airway Diseases.Mediators of inflammation · 2015Review
- Altered Mucins (MUC) trafficking in benign and malignant conditions.Oncotarget · 2014Review
- Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF-/- mice.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Regulated mucin secretion from airway epithelial cells.Frontiers in endocrinology · 2013Review
- Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- IL-13-induced airway mucus production is attenuated by MAPK13 inhibition.The Journal of clinical investigation · 2012Article
- Calcium-activated chloride channel TMEM16A modulates mucin secretion and airway smooth muscle contraction.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundExcess mucus in the airways leads to obstruction in diseases such as chronic bronchitis, asthma, and cystic fibrosis. Mucins, the highly glycosolated protein components of mucus, are stored in membrane-bound granules housed in the cytoplasm of airway epithelial "goblet" cells until they are secreted into the airway lumen via an exocytotic process. Precise mechanism(s) of mucin secretion, including the specific proteins involved in the process, have yet to be elucidated. Previously, we have shown that the Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) protein regulates mucin secretion by orchestrating translocation of mucin granules from the cytosol to the plasma membrane, where the granules dock, fuse and release their contents into the airway lumen. Associated with MARCKS in this process are chaperone (Heat Shock Protein 70 [HSP70], Cysteine string protein [CSP]) and cytoskeletal (actin, myosin) proteins. However, additional granule-associated proteins that may be involved in secretion have not yet been elucidated.
methodsHere, we isolated mucin granules and granule membranes from primary cultures of well differentiated human bronchial epithelial cells utilizing a novel technique of immuno-isolation, based on the presence of the calcium activated chloride channel hCLCA1 (the human ortholog of murine Gob-5) on the granule membranes, and verified via Western blotting and co-immunoprecipitation that MARCKS, HSP70, CSP and hCLCA1 were present on the granule membranes and associated with each other. We then subjected the isolated granules/membranes to liquid chromatography mass spectrometry (LC-MS/MS) to identify other granule associated proteins.
resultsA number of additional cytoskeletal (e.g. Myosin Vc) and regulatory proteins (e.g. Protein phosphatase 4) associated with the granules and could play a role in secretion were discovered. This is the first description of the airway goblet cell "granulome."
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