ArticleThe Biochemical journal2011
Intracellular curvature-generating proteins in cell-to-cell fusion.
Article in The Biochemical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The trial behind it
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Who cites it
32 citing papers in PubMed, 40 citations in OpenAlex.
- FCHO1 fine-tunes synaptic vesicle endocytosis in an activity-dependent manner.Molecular brain · 2026Article
- Cell-cell fusion: To lose one life and begin another.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Novel requirements for HAP2/GCS1-mediated gamete fusion iniScience · 2024Article
- Regulation of the myoblast fusion reaction for muscle development, regeneration, and adaptations.Experimental cell research · 2022Article
- Binucleated and Multinucleated Neurons are Formed by Fusion.Bulletin of experimental biology and medicine · 2021Article
- Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein.Nature cell biology · 2020Article
- Inhibition of influenza virus activity by the bovine seminal plasma protein PDC-109.European biophysics journal : EBJ · 2019Article
- RNAi Screen inG3 (Bethesda, Md.) · 2019Article
- Cell-surface phosphatidylserine regulates osteoclast precursor fusion.The Journal of biological chemistry · 2018Article
- Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion.The Journal of cell biology · 2017Article
- MFN1 structures reveal nucleotide-triggered dimerization critical for mitochondrial fusion.Nature · 2017Article
- Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis.EMBO reports · 2016Article
- An Amphiphysin-Like Domain in Fus2p Is Required for Rvs161p Interaction and Cortical Localization.G3 (Bethesda, Md.) · 2015Article
- Membrane tension and membrane fusion.Current opinion in structural biology · 2015Review
- Reovirus FAST Proteins Drive Pore Formation and Syncytiogenesis Using a Novel Helix-Loop-Helix Fusion-Inducing Lipid Packing Sensor.PLoS pathogens · 2015Article
- Analytical characterization of the role of phospholipids in platelet adhesion and secretion.Analytical chemistry · 2015Article
- Late stages of the synchronized macrophage fusion in osteoclast formation depend on dynamin.The Biochemical journal · 2014Article
- Dynamin and endocytosis are required for the fusion of osteoclasts and myoblasts.The Journal of cell biology · 2014Article
- GRAF1 promotes ferlin-dependent myoblast fusion.Developmental biology · 2014Article
- Efficient reovirus- and measles virus-mediated pore expansion during syncytium formation is dependent on annexin A1 and intracellular calcium.Journal of virology · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Cell-to-cell fusion plays an important role in normal physiology and in different pathological conditions. Early fusion stages mediated by specialized proteins and yielding fusion pores are followed by a pore expansion stage that is dependent on cell metabolism and yet unidentified machinery. Because of a similarity of membrane bending in the fusion pore rim and in highly curved intracellular membrane compartments, in the present study we explored whether changes in the activity of the proteins that generate these compartments affect cell fusion initiated by protein fusogens of influenza virus and baculovirus. We raised the intracellular concentration of curvature-generating proteins in cells by either expressing or microinjecting the ENTH (epsin N-terminal homology) domain of epsin or by expressing the GRAF1 (GTPase regulator associated with focal adhesion kinase 1) BAR (Bin/amphiphysin/Rvs) domain or the FCHo2 (FCH domain-only protein 2) F-BAR domain. Each of these treatments promoted syncytium formation. Cell fusion extents were also influenced by treatments targeting the function of another curvature-generating protein, dynamin. Cell-membrane-permeant inhibitors of dynamin GTPase blocked expansion of fusion pores and dominant-negative mutants of dynamin influenced the syncytium formation extents. We also report that syncytium formation is inhibited by reagents lowering the content and accessibility of PtdIns(4,5)P(2), an important regulator of intracellular membrane remodelling. Our findings indicate that fusion pore expansion at late stages of cell-to-cell fusion is mediated, directly or indirectly, by intracellular membrane-shaping proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.