ArticleJournal of biomedical optics2011
Genetically encoded ratiometric biosensors to measure intracellular exchangeable zinc in Escherichia coli.
Article in Journal of biomedical optics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 55 citations in OpenAlex.
- Mechanism of Kch activation by ZnPNAS nexus · 2026Article
- Bacterial Metallostasis: Metal Sensing, Metalloproteome Remodeling, and Metal Trafficking.Chemical reviews · 2024Review
- Structures, Mechanisms, and Physiological Functions of Zinc Transporters in Different Biological Kingdoms.International journal of molecular sciences · 2024Review
- Metal retention and replacement in QueD2 protect queuosine-tRNA biosynthesis in metal-starvedProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response.International journal of molecular sciences · 2022Article
- Zur: Zinc-Sensing Transcriptional Regulator in a Diverse Set of Bacterial Species.Pathogens (Basel, Switzerland) · 2021Review
- Combining Active Carbonic Anhydrase with Nanogels: Enzyme Protection and Zinc Sensing.International journal of nanomedicine · 2021Article
- Metallochaperones Are Needed for Mycobacterium tuberculosis and Escherichia coli Nicotinamidase-Pyrazinamidase Activity.Journal of bacteriology · 2020Article
- The Ribosome as a Switchboard for Bacterial Stress Response.Frontiers in microbiology · 2020Review
- Allosteric histidine switch for regulation of intracellular zinc(II) fluctuation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The structural basis of proton driven zinc transport by ZntB.Nature communications · 2017Article
- Microbial Carbonic Anhydrases in Biomimetic Carbon Sequestration for Mitigating Global Warming: Prospects and Perspectives.Frontiers in microbiology · 2017Review
- Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution.Nature chemical biology · 2016Article
- Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc.ACS chemical biology · 2016Article
- High-resolution in vivo optical imaging of stroke injury and repair.Brain research · 2015Review
- Zinc regulates a switch between primary and alternative S18 ribosomal proteins in Mycobacterium tuberculosis.Molecular microbiology · 2015Article
- The capability of Pseudomonas aeruginosa to recruit zinc under conditions of limited metal availability is affected by inactivation of the ZnuABC transporter.Metallomics : integrated biometal science · 2015Article
- The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress.Journal of the Royal Society, Interface · 2015Article
- Kinetics and thermodynamics of metal-binding to histone deacetylase 8.Protein science : a publication of the Protein Society · 2015Article
- Bacillithiol is a major buffer of the labile zinc pool in Bacillus subtilis.Molecular microbiology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Zinc is an essential element for numerous cellular processes, therefore zinc homeostasis is regulated in living organisms. Fluorescent sensors have been developed as important tools to monitor the concentrations of readily exchangeable zinc in live cells. One type of biosensor uses carbonic anhydrase (CA) as the recognition element based on its tunable affinity, superior metal selectivity, and fluorescence signal from aryl sulfonamide ligands coupled to zinc binding. Here, we fuse carbonic anhydrase with a red fluorescent protein to create a series of genetically-encoded Förster resonance energy transfer-based excitation ratiometric zinc sensors that exhibit large signal increases in response to alterations in physiological-free zinc concentrations. These sensors were applied to the prokaryotic model organism Escherichia coli to quantify the readily exchangeable zinc concentration. In minimal media, E. coli BL21(DE3) cells expressing the CA sensor, exhibit a median intracellular readily exchangeable zinc concentration of 20 pM, much less than the total cellular zinc concentration of ∼0.2 mM. Furthermore, the intracellular readily exchangeable zinc concentration varies with the concentration of environmental zinc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.