ArticleJournal of the American Chemical Society2011
N-terminal protein modification using simple aminoacyl transferase substrates.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Design principles and functional basis of enantioselectivity of alanyl-tRNA synthetase and a chiral proofreader during protein biosynthesis.Nucleic acids research · 2023Article
- Development of New Tools for the Studies of Protein Arginylation.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Bypassing the requirement for aminoacyl-tRNA by a cyclodipeptide synthase enzyme.RSC chemical biology · 2021Article
- The chemistry and applications of RNA 2'-OH acylation.Nature reviews. Chemistry · 2020Article
- Single-site glycine-specific labeling of proteins.Nature communications · 2019Article
- Use of the dehydrophos biosynthetic enzymes to prepare antimicrobial analogs of alaphosphin.Organic & biomolecular chemistry · 2019Article
- Biochemical analysis of protein arginylation.Methods in enzymology · 2019Article
- Selective N-terminal functionalization of native peptides and proteins.Chemical science · 2017Article
- Minimalist IR and fluorescence probes of protein function.Current opinion in chemical biology · 2016Review
- Non-canonical roles of tRNAs and tRNA mimics in bacterial cell biology.Molecular microbiology · 2016Review
- Chemoselective modifications for the traceless ligation of thioamide-containing peptides and proteins.Organic & biomolecular chemistry · 2016Article
- Comparison of strategies for non-perturbing labeling of α-synuclein to study amyloidogenesis.Organic & biomolecular chemistry · 2016Article
- Perspectives and Insights into the Competition for Aminoacyl-tRNAs between the Translational Machinery and for tRNA Dependent Non-Ribosomal Peptide Bond Formation.Life (Basel, Switzerland) · 2015Review
- A peptide ligase and the ribosome cooperate to synthesize the peptide pheganomycin.Nature chemical biology · 2015Article
- Specific modulation of protein activity by using a bioorthogonal reaction.Chembiochem : a European journal of chemical biology · 2014Article
- The determination of tRNALeu recognition nucleotides for Escherichia coli L/F transferase.RNA (New York, N.Y.) · 2014Article
- A general method for fluorescent labeling of the N-termini of lanthipeptides and its application to visualize their cellular localization.Journal of the American Chemical Society · 2013Article
- Expressed protein ligation at methionine: N-terminal attachment of homocysteine, ligation, and masking.Angewandte Chemie (International ed. in English) · 2013Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Methods for synthetically manipulating protein structure enable greater flexibility in the study of protein function. Previous characterization of the Escherichia coli aminoacyl tRNA transferase (AaT) has shown that it can modify the N-terminus of a protein with an amino acid from a tRNA or a synthetic oligonucleotide donor. Here, we demonstrate that AaT can efficiently use a minimal adenosine substrate, which can be synthesized in one to two steps from readily available starting materials. We have characterized the enzymatic activity of AaT with aminoacyl adenosyl donors and found that reaction products do not inhibit AaT. The use of adenosyl donors removes the substrate limitations imposed by the use of synthetases for tRNA charging and avoids the complex synthesis of an oligonucleotide donor. Thus, our AaT donors increase the potential substrate scope and reaction scale for N-terminal protein modification under conditions that maintain folding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.