ArticleCell research2011
Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis.
Article in Cell research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 92 citations in OpenAlex.
- Article
- Glycan Modifications as Regulators of Stem Cell Fate.Biology · 2024Review
- Characterization of Cell Surface Glycan Profiles in Human and Mouse Corneas Using Lectin Microarrays.Cells · 2023Article
- Glycans in spent embryo culture medium are related to the implantation ability of blastocysts.Heliyon · 2023Article
- Unlocking the Pragmatic Potential of Regenerative Therapies in Heart Failure with Next-Generation Treatments.Biomedicines · 2023Review
- Optimizing Generation of Stem Cell-Derived Islet Cells.Stem cell reviews and reports · 2022Review
- Downstream bioprocessing of human pluripotent stem cell-derived therapeutics.Engineering in life sciences · 2022Review
- Deficiency of N-glycanase 1 perturbs neurogenesis and cerebral development modeled by human organoids.Cell death & disease · 2022Article
- Glycan detecting tools developed from the Clostridium botulinum whole hemagglutinin complex.Scientific reports · 2021Article
- Rewinding Extinction in the Northern White Rhinoceros: Genetically Diverse Induced Pluripotent Stem Cell Bank for Genetic Rescue.Stem cells and development · 2021Article
- The Present State and Future Perspectives of Cardiac Regenerative Therapy Using Human Pluripotent Stem Cells.Frontiers in cardiovascular medicine · 2021Review
- GABAergic System Dysfunction and Challenges in Schizophrenia Research.Frontiers in cell and developmental biology · 2021Review
- Embryonic Stem Cells in Clinical Trials: Current Overview of Developments and Challenges.Advances in experimental medicine and biology · 2021Review
- Lectin drug conjugate therapy for colorectal cancer.Cancer science · 2020Article
- Cell-Based Therapeutic Approaches for Cystic Fibrosis.International journal of molecular sciences · 2020Review
- Prevention of tumor risk associated with the reprogramming of human pluripotent stem cells.Journal of experimental & clinical cancer research : CR · 2020Review
- Articular chondrocytes from osteoarthritic knee joints of elderly,Regenerative therapy · 2020Article
- A technique for removing tumourigenic pluripotent stem cells using rBC2LCN lectin.Regenerative therapy · 2020Article
- Urine Sample-Derived Cerebral Organoids Suitable for Studying Neurodevelopment and Pharmacological Responses.Frontiers in cell and developmental biology · 2020Article
- iPS-Cell Technology and the Problem of Genetic Instability-Can It Ever Be Safe for Clinical Use?Journal of clinical medicine · 2019Review
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15 authors at 3 institutions in 2 countries.
Funding
Abstract
Rapid and dependable methods for isolating human pluripotent stem cell (hPSC) populations are urgently needed for quality control in basic research and in cell-based therapy applications. Using lectin arrays, we analyzed glycoproteins extracted from 26 hPSC samples and 22 differentiated cell samples, and identified a small group of lectins with distinctive binding signatures that were sufficient to distinguish hPSCs from a variety of non-pluripotent cell types. These specific biomarkers were shared by all the 12 human embryonic stem cell and the 14 human induced pluripotent stem cell samples examined, regardless of the laboratory of origin, the culture conditions, the somatic cell type reprogrammed, or the reprogramming method used. We demonstrated a practical application of specific lectin binding by detecting hPSCs within a differentiated cell population with lectin-mediated staining followed by fluorescence microscopy and flow cytometry, and by enriching and purging viable hPSCs from mixed cell populations using lectin-mediated cell separation. Global gene expression analysis showed pluripotency-associated differential expression of specific fucosyltransferases and sialyltransferases, which may underlie these differences in protein glycosylation and lectin binding. Taken together, our results show that protein glycosylation differs considerably between pluripotent and non-pluripotent cells, and demonstrate that lectins may be used as biomarkers to monitor pluripotency in stem cell populations and for removal of viable hPSCs from mixed cell populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.